- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA042Mu
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- 详细信息
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Mus musculus (Mouse，小鼠)
粒细胞集落刺激因子(GCSF)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 3.2pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
粒细胞集落刺激因子(GCSF)检测试剂盒
yb-E12525 大鼠血清淀粉样蛋白A(SAA)ELISA试剂盒 Serum amyloid A (SAA) ELISA kit
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yb-E12527 大鼠α1酸性糖蛋白(α1-AGP)ELISA试剂盒 Rat α1 -acid glycoprotein (α1-AGP) ELISA Kit
yb-E12528 大鼠内皮型一氧化氮合成酶3(eNOS-3)ELISA试剂盒 Rat endothelial nitric oxide synthase 3 (eNOS-3) ELISA kit
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yb-E12532 大鼠丙二醛(MDA)ELISA试剂盒 Malondialdehyde (MDA) ELISA kit
yb-E12533 大鼠超氧化物歧化酶(SOD)ELISA试剂盒 Rat superoxide dismutase (SOD) ELISA kit
yb-E12534 大鼠热休克蛋白糖蛋白96(HSP gp96)ELISA试剂盒 Heat shock proteins in rat glycoprotein 96 (HSP gp96) ELISA kit
yb-E12535 大鼠血清总补体(CH50)ELISA试剂盒 Serum total complement (CH50) ELISA kit
yb-E12536 大鼠可溶性CD86(B7-2/sCD86)ELISA试剂盒 Rat soluble CD86 (B7-2/sCD86) ELISA Kit
yb-E12537 大鼠低氧诱导因子1α(HIF-1α)ELISA试剂盒 Rat hypoxia-inducible factor 1α (HIF-1α) ELISA kit
yb-E12538 大鼠胰淀素(Amylin)ELISA试剂盒 Rat amylin (Amylin) ELISA kit
yb-E12539 大鼠白三烯C4(LTC4)ELISA试剂盒 Rat leukotriene C4 (LTC4) ELISA kit
yb-E12540 大鼠细胞色素C(Cyt-C)ELISA试剂盒 Rat cytochrome C (Cyt-C) ELISA kit
yb-E12541 大鼠血管紧张素Ⅰ(Ang-Ⅰ)ELISA试剂盒 Rat Angiotensin Ⅰ (Ang-Ⅰ) ELISA kit
yb-E12542 大鼠黑色素细胞抗体(MC Ab)ELISA试剂盒 Melanocytes rat antibody (MC Ab) ELISA Kit
yb-E12543 大鼠二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒 Rats dipeptidyl peptidase Ⅳ (DPP Ⅳ) ELISA Kit
yb-E12544 大鼠过敏毒素/补体片断4a(C4a)ELISA试剂盒 Rat Anaphylatoxin / complement fragment 4a (C4a) ELISA kit
yb-E12545 大鼠补体片断5a(C5a)ELISA试剂盒 Fragment of rat complement 5a (C5a) ELISA Kit
yb-E12546 大鼠补体片断3a(C3a)ELISA试剂盒 Rat complement fragment 3a (C3a) ELISA Kit
粒细胞集落刺激因子(GCSF)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 3.2pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
粒细胞集落刺激因子(GCSF)检测试剂盒
ELISA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | SEA042Mu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 7.81-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL, 7.81pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 3.2pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 3, Granulocyte (GCSF).
No significant cross-reactivity or interference between Colony Stimulating Factor 3, Granulocyte (GCSF) and analogues was observed.
No significant cross-reactivity or interference between Colony Stimulating Factor 3, Granulocyte (GCSF) and analogues was observed.
Recovery
粒细胞集落刺激因子(GCSF)检测试剂盒Matrices listed below were spiked with certain level of recombinant Colony Stimulating Factor 3, Granulocyte (GCSF) and the recovery rates were calculated by comparing the measured value to the expected amount of Colony Stimulating Factor 3, Granulocyte (GCSF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-104 | 98 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 87-101 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 3, Granulocyte (GCSF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 3, Granulocyte (GCSF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 3, Granulocyte (GCSF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Colony Stimulating Factor 3, Granulocyte (GCSF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 83-97% | 81-88% | 80-89% |
| EDTA plasma(n=5) | 90-103% | 97-105% | 94-103% | 89-102% |
| heparin plasma(n=5) | 80-104% | 88-97% | 78-101% | 87-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
粒细胞集落刺激因子(GCSF)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 3, Granulocyte (GCSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 3, Granulocyte (GCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 3, Granulocyte (GCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 3, Granulocyte (GCSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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yb-E12545 大鼠补体片断5a(C5a)ELISA试剂盒 Fragment of rat complement 5a (C5a) ELISA Kit
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粒细胞集落刺激因子(GCSF)检测试剂盒
¥900 - 1890
