- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEB028Rb
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Oryctolagus cuniculus (Rabbit，兔)
白蛋白(ALB)检测试剂盒
检测范围 617.28-50000ng/mL 灵敏度 245.5ng/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
白蛋白(ALB)检测试剂盒
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yb-E13334 兔子白介素9(IL-9)ELISA试剂盒 Rabbit interleukin 9 (IL-9) ELISA Kit
yb-E13335 兔载脂蛋白A1(apo-A1)ELISA试剂盒 Rabbit apolipoprotein A1 (apo-A1) ELISA Kit
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yb-E13339 兔血浆α颗粒膜蛋白(GMP-140)ELISA试剂盒 Rabbit plasma α granule membrane protein (GMP-140) ELISA kit
yb-E13340 兔一氧化氮(NO)ELISA试剂盒 Rabbit NO (NO) ELISA Kit
yb-E13341 兔补体蛋白3(C3)ELISA试剂盒 Rabbit complement protein 3 (C3) ELISA Kit
yb-E13342 兔p物质(SP)ELISA试剂盒 Rabbit p substance (SP) ELISA kit
yb-E13361 兔子生长激素释放多肽(GHRP)ELISA试剂盒 Rabbit growth hormone releasing peptide (GHRP) ELISA Kit
yb-E13362 兔子α干扰素(IFN-α)ELISA试剂盒 Rabbit α interferon (IFN-α) ELISA kit
yb-E13363 兔子E选择素(E-Selectin/CD62E)ELISA试剂盒 Rabbit E -selectin (E-Selectin/CD62E) ELISA kit
yb-E13364 兔子视黄醇结合蛋白4(RBP-4)ELISA试剂盒 Rabbit retinol binding protein 4 (RBP-4) ELISA kit
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白蛋白(ALB)检测试剂盒
检测范围 617.28-50000ng/mL 灵敏度 245.5ng/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
白蛋白(ALB)检测试剂盒
ELISA Kit for Albumin (ALB)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Oryctolagus cuniculus (Rabbit) |
| Product No. | CEB028Rb |
| Sample type | Serum, plasma and other biological fluids. |
| Format | 96T |
| Assay length | 2.5 hours |
| Detection range | 617.28-50000ng/mL The standard curve concentrations used for the ELISA’s were 50000ng/mL, 16666.67ng/mL, 5555.56ng/mL, 1851.85ng/mL, 617.28ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 245.5ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-103 | 99 |
| EDTA plasma(n=5) | 82-101 | 97 |
| heparin plasma(n=5) | 88-96 | 93 |
Precision
白蛋白(ALB)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 79-103% | 91-105% | 85-101% |
| EDTA plasma(n=5) | 98-105% | 78-102% | 95-104% | 78-103% |
| heparin plasma(n=5) | 79-94% | 78-95% | 86-96% | 94-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
白蛋白(ALB)检测试剂盒This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Albumin (ALB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Albumin (ALB) and unlabeled Albumin (ALB) (Standards or samples) with the pre-coated antibody specific to Albumin (ALB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Albumin (ALB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Albumin (ALB) in the sample.
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yb-E13336 兔纤溶酶抗纤溶酶复合物(PAP)ELISA试剂盒 Rabbit plasmin -antiplasmin complexes (PAP) ELISA Kit
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yb-E13366 兔淋巴细胞功能相关抗原3(LFA-3/CD58)ELISA试剂盒 Rabbit lymphocyte function -associated antigen 3 (LFA-3/CD58) ELISA kit
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白蛋白(ALB)检测试剂盒
¥900 - 1890
