- ¥1580 - 1980
- 上海钰博
- 见说明书
- 德国/美国/中国
- ybEB409Hu
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
钰博生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
产品编号 ybEB409Hu 适用生物 Homo sapiens (Human，人)
血红蛋白(HB)检测试剂盒
检测范围 1.23-100μg/mL 灵敏度 0.57μg/mL
样本类型 Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
yb-E10009 人磷脂酶A2（PL-A2）ELISA试剂盒 Human phospholipase A2 (PL-A2) ELISA Kit
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yb-E10011 人凝血酶原片段F1+2(F1+2)ELISA试剂盒 Human prothrombin fragment F1 +2 (F1 +2) ELISA Kit
yb-E10012 人β淀粉样蛋白42（ Aβ-42）ELISA试剂盒 Human β-amyloid 42 (Aβ-42) ELISA Kit
yb-E10013 人β淀粉样蛋白40（ Aβ-40）ELISA试剂盒 Human β-amyloid 40 (Aβ-40) ELISA kit
yb-E10014 人整合素αⅤβ1（ITG αⅤβ1）ELISA试剂盒 Human integrin α Ⅴ β1 (ITG α Ⅴ β1) ELISA kit
yb-E10015 人亚麻酸（LA）ELISA试剂盒 Human linolenic (LA) ELISA Kit
yb-E10016 人神经元特异性烯化醇ELISA试剂盒 Human neuron-specific ELISA kit allyl alcohol
yb-E10017 人神经元特异性烯醇化酶(NSE)ELISA试剂盒 Human neuron-specific enolase (NSE) ELISA kit
yb-E10018 人血吸虫ELISA试剂盒 Human schistosome ELISA kit
yb-E10019 人维生素B6ELISA试剂盒 Human vitamin B6ELISA kit
yb-E10020 人正五聚蛋白3（PTX3）ELISA试剂盒 Human pentraxin 3 (PTX3) ELISA kit
yb-E10021 人维生素B9ELISA试剂盒 Human vitamin B9ELISA kit
yb-E10022 人维生素B12 ELISA试剂盒 Human vitamin B12 ELISA Kit
yb-E10023 人纤维蛋白原降解产物（FDP)ELISA试剂盒 Fibrinogen degradation products (FDP) ELISA Kit
yb-E10024 人半胱氨酸蛋白酶抑制剂B（CSTB)ELISA试剂盒 Human cystatin B (CSTB) ELISA Kit
yb-E10025 人雌酮(E1)ELISA试剂盒 Human estrone (E1) ELISA kit
yb-E10026 人二氢睾酮（DHT）ELISA试剂盒 People dihydrotestosterone (DHT) ELISA kit
yb-E10027 人游离睾酮(F-TESTO)ELISA试剂盒 People free testosterone (F-TESTO) ELISA kit
yb-E10028 人神经肽S(NPS)ELISA试剂盒 Human neuropeptide S (NPS) ELISA kit
yb-E10029 人神经肽S受体(NPSR）ELISA试剂盒 Human neuropeptide S receptor (NPSR) ELISA kit
yb-E10030 人磷脂酰肌醇蛋白聚糖3（GPC-3）ELISA试剂盒 Phospholipicl glypican 3 (GPC-3) ELISA Kit
yb-E10031 人血管性血友病因子 (vWF)ELISA试剂盒 Human von Willebrand factor (vWF) ELISA kit
yb-E10032 人突触核蛋白a(SNCa）ELISA试剂盒 Human synuclein a (SNCa) ELISA Kit
yb-E10033 人总抗氧化状态(TOS)ELISA试剂盒 People total antioxidant status (TOS) ELISA kit
yb-E10034 人免疫球蛋白A Fc段受体Ⅰ(FcαRⅠ/CD89)ELISA试剂盒Segmentof human immunoglobulinA FcreceptorⅠ (FcαR Ⅰ / CD89) ELISAKit
yb-E10035 人免疫球蛋白E Fc段受体Ⅱ(FcεRⅡ/CD23)ELISA试剂盒Segmentof human immunoglobulinE FcreceptorⅡ (FcεR Ⅱ / CD23) ELISAKit
血红蛋白(HB)检测试剂盒
检测范围 1.23-100μg/mL 灵敏度 0.57μg/mL
样本类型 Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
ELISA Kit for Hemoglobin (HB)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | CEB409Hu |
| Sample type | Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 2.5 hours |
| Detection range | 1.23-100μg/mL The standard curve concentrations used for the ELISA’s were 100μg/mL, 33.33μg/mL, 11.11μg/mL, 3.7μg/mL, 1.23μg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.57μg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Hemoglobin (HB).
No significant cross-reactivity or interference between Hemoglobin (HB) and analogues was observed.
No significant cross-reactivity or interference between Hemoglobin (HB) and analogues was observed.
Recovery
血红蛋白(HB)检测试剂盒 Matrices listed below were spiked with certain level of recombinant Hemoglobin (HB) and the recovery rates were calculated by comparing the measured value to the expected amount of Hemoglobin (HB) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-103 | 101 |
| EDTA plasma(n=5) | 79-105 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin (HB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin (HB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin (HB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hemoglobin (HB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 95-103% | 80-92% | 86-103% |
| EDTA plasma(n=5) | 96-105% | 79-105% | 92-101% | 88-99% |
Stability
血红蛋白(HB)检测试剂盒 The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
血红蛋白(HB)检测试剂盒 This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin (HB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin (HB) and unlabeled Hemoglobin (HB) (Standards or samples) with the pre-coated antibody specific to Hemoglobin (HB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin (HB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin (HB) in the sample.
yb-E10008 人骨特异性碱性磷酸酶B(ALP-B)ELISA试剂盒 Human bone-specific alkaline phosphatase B (ALP-B) ELISA kit
yb-E10009 人磷脂酶A2（PL-A2）ELISA试剂盒 Human phospholipase A2 (PL-A2) ELISA Kit
yb-E10010 人硫胺素（Thiamine）ELISA试剂盒 People thiamine (Thiamine) ELISA kit
yb-E10011 人凝血酶原片段F1+2(F1+2)ELISA试剂盒 Human prothrombin fragment F1 +2 (F1 +2) ELISA Kit
yb-E10012 人β淀粉样蛋白42（ Aβ-42）ELISA试剂盒 Human β-amyloid 42 (Aβ-42) ELISA Kit
yb-E10013 人β淀粉样蛋白40（ Aβ-40）ELISA试剂盒 Human β-amyloid 40 (Aβ-40) ELISA kit
yb-E10014 人整合素αⅤβ1（ITG αⅤβ1）ELISA试剂盒 Human integrin α Ⅴ β1 (ITG α Ⅴ β1) ELISA kit
yb-E10015 人亚麻酸（LA）ELISA试剂盒 Human linolenic (LA) ELISA Kit
yb-E10016 人神经元特异性烯化醇ELISA试剂盒 Human neuron-specific ELISA kit allyl alcohol
yb-E10017 人神经元特异性烯醇化酶(NSE)ELISA试剂盒 Human neuron-specific enolase (NSE) ELISA kit
yb-E10018 人血吸虫ELISA试剂盒 Human schistosome ELISA kit
yb-E10019 人维生素B6ELISA试剂盒 Human vitamin B6ELISA kit
yb-E10020 人正五聚蛋白3（PTX3）ELISA试剂盒 Human pentraxin 3 (PTX3) ELISA kit
yb-E10021 人维生素B9ELISA试剂盒 Human vitamin B9ELISA kit
yb-E10022 人维生素B12 ELISA试剂盒 Human vitamin B12 ELISA Kit
yb-E10023 人纤维蛋白原降解产物（FDP)ELISA试剂盒 Fibrinogen degradation products (FDP) ELISA Kit
yb-E10024 人半胱氨酸蛋白酶抑制剂B（CSTB)ELISA试剂盒 Human cystatin B (CSTB) ELISA Kit
yb-E10025 人雌酮(E1)ELISA试剂盒 Human estrone (E1) ELISA kit
yb-E10026 人二氢睾酮（DHT）ELISA试剂盒 People dihydrotestosterone (DHT) ELISA kit
yb-E10027 人游离睾酮(F-TESTO)ELISA试剂盒 People free testosterone (F-TESTO) ELISA kit
yb-E10028 人神经肽S(NPS)ELISA试剂盒 Human neuropeptide S (NPS) ELISA kit
yb-E10029 人神经肽S受体(NPSR）ELISA试剂盒 Human neuropeptide S receptor (NPSR) ELISA kit
yb-E10030 人磷脂酰肌醇蛋白聚糖3（GPC-3）ELISA试剂盒 Phospholipicl glypican 3 (GPC-3) ELISA Kit
yb-E10031 人血管性血友病因子 (vWF)ELISA试剂盒 Human von Willebrand factor (vWF) ELISA kit
yb-E10032 人突触核蛋白a(SNCa）ELISA试剂盒 Human synuclein a (SNCa) ELISA Kit
yb-E10033 人总抗氧化状态(TOS)ELISA试剂盒 People total antioxidant status (TOS) ELISA kit
yb-E10034 人免疫球蛋白A Fc段受体Ⅰ(FcαRⅠ/CD89)ELISA试剂盒Segmentof human immunoglobulinA FcreceptorⅠ (FcαR Ⅰ / CD89) ELISAKit
yb-E10035 人免疫球蛋白E Fc段受体Ⅱ(FcεRⅡ/CD23)ELISA试剂盒Segmentof human immunoglobulinE FcreceptorⅡ (FcεR Ⅱ / CD23) ELISAKit
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【医学检验技士】 医药卫生 >> 卫生职称 >> 临床医学检验技士 血红蛋白（Hb）测定 考试重点、难点名词 血红蛋白正常范围： 男性120～160g／L（12.0-16.0g／dl）； 女性110～150g／L（11.0-15.0g／dl）； 新生儿170～200g／L（18.0-19.0g／dl）。 检查介绍：血红蛋白又称血色素，是红细胞的主要组成部分，能与氧结合
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血红蛋白(HB)检测试剂盒
¥1580 - 1980
