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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Ctenocephalides felis Salivary antigen 1
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥17754.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4956.0 |
| 规格: | 20μg | 产品价格: | ¥2616.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
/Uniprot No.:
Q94424别名:
FS-I Allergen: Cte f 1种属:
Ctenocephalides felis (Cat flea)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
20.1 kDa表达区域:
19-176aa氨基酸序列:
EDIWKVNKKCTSGGKNQDRKLDQIIQKGQQVKIQNICKLIRDKPHTNQEKEKCMKFCKKVCKGYRGACDGNICYCSRPSNLGPDWKVSKECKDPNNKDSRPTEIVPYRQQLAIPNICKLKNSETNEDSKCKKHCKEKCRGGNDAGCDGNFCYCRPKNK蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Salivary antigens of Ctenocephalides felis: collection, purification and evaluation by intradermal skin testing in dogs."Frank G.R., Hunter S.W., Wallenfels L.J., Kwochka K.(In) Kwochka K., Von Tscharner C., Willemse T. (eds.); Advances in veterinary dermatology, pp.3:201-212, Butterworth-Heinemann Medical, Oxford (1998)Function:
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文献和实验of the salivary proteome as a surrogate tissue for systems biology approaches to understanding appetite. J. Proteomics 75:2916‐2923. He, L., Li, P., Sha, Y.Q., Chen, Z.B., Luan, Q.X
肝炎病毒包膜E2蛋白相同的亲和结合力3。另外,有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4) 利用Rho1D4纯化膜蛋白图解简述步骤1:结合:将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2:清洗:将杂蛋白和其他裂解液成分洗去,留下与亲和基质结合的目标蛋白。 步骤3:洗脱:过量Rho1D4肽与基质竞争性结合,释放出目标蛋白在洗脱液中收集。 表
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
]Mougeotia scalaris recombinant phytochromeI, 100 mg/L, active470Plasmodium falciparum merozoite surface protein 1 (MSP-1)S, 24 mg/L, α-MF[93]Plasmodium falciparum antigen Pfs48/45I (1% total protein) or S, α-MF, inactive448Plasmodium falciparum antigen P30P2
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