CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
ScienCell's human CD40 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CD40-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human CD40 in the sample that is captured on the strips.
Catalog No.EK0702Country of ManufactureUnited StatesProduct CodehCD40-ELISASize/Quantity96 testsProduct UseThis product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.StorageStore at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.Shipping InfoShipped on gel iceReferences1. Clark, E. A. CD40: a cytokine receptor in search of a ligand. Tissue Antigens 35: 33-36, 1990.
2. Lafage-Pochitaloff, M., Herman, P., Birg,F., Galizzi, J.-P., Simonetti, J., Mannoni, P., Banchereau, J. Localization of the human CD40 gene to chromosome 20, bands q12-q13.2. Leukemia 8: 1172-1175, 1994.
3. "Entrez Gene: CD40 CD40 molecule, TNF receptor superfamily member 5".