小鼠EGF蛋白

小鼠EGF蛋白
产品信息
别称：
Epidermal Growth Factor
蛋白构建：
A DNA sequence encoding the mouse EGF (P01132) (Asn977-Arg1029) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
种属：
Mouse
表达宿主：
Human cells
产品状态&运输说明：
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

小鼠EGF蛋白
质控检测
纯度：
> 95 % as determined by SDS-PAGE.
生物活性：
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is typically 0.1-0.6 ng/mL.

内毒素：
< 1.0 EU per μg of the protein as determined by the LAL method
稳定性：
Samples are stable for up to twelve months from date of receipt at -70℃
预测N端：
Gly
分子量：
The recombinant mouse EGF consists of 55 amino acids and predicts a molecular mass of 6.2 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
小鼠EGF蛋白
缓冲液：
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.


使用指导
储存方法：
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
小鼠EGF蛋白
复溶条件：
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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