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- 详细信息
- 文献和实验
- 技术资料
- 库存：
2-3周
- 供应商：
研卉生物
- 检测方法：
ELISA
- 规格：
96T
Human Cu/ZnSOD Platinum ELISA
Also known as: Cu/Zn Superoxide Dismutase
RUO: For Research Use Only. Not for use in diagnostic procedures.
Cat. No. Size
BMS222 96 tests
BMS222TEN 10 x 96 tests
Description: The human Cu/ZnSOD ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Cu/ZnSOD. The human Cu/ZnSOD ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Superoxide Dismutases (SODs) are a unique family of metalloproteins that catalyze the dismutation of superoxide anion radicals (O2-) to oxygen (O2) and hydrogen peroxide (H2O2)
O2- + O2- + 2H+ => H2O2 + O2
SOD is ubiquitous in oxygen metabolizing cells protecting these cells against direct and indirect oxygen-mediated free radical damage. Four types of SOD have been defined on the basis of distinctions in their metal cofactors and distribution: Manganese (MnSOD) principally located in the matrix of mitochondria of all aerobes, copper/zinc (Cu/ZnSOD) mainly present in the cytoplasm of eukaryotic cells, iron (FeSOD), predominantly in the cytosol, chloropasts or mitochondria of prokaryotes as well as extracellular (ECSOD), which is found in the extracellular fluids or membrane associated in mammals. The properties of Cu/Zn superoxide dismutase are quite different from those of the manganese or iron enzymes.
Induction of Cu/ZnSOD expression resulting in elevated levels of Cu/ZnSOD in human body fluids is of diagnostic value for measuring the activity of different diseases:
Nephropathies
Cu/ZnSOD determination provides a tool for early diagnosis of nephropathies.
Monitoring of therapeutic treatments
Cu/ZnSOD is a useful therapeutic tool in the treatment of chronic inflammation e.g. rheumatoid arthritis or of the ischemic myocardium in the phase of reperfusion. Due to the short half-life of SOD injected into the blood circulation, a rapid assay is necessary for monitoring SOD levels.
Trisomy 21 (Down's Syndrome)
In cases with Down's Syndrome an additional part of chromosome 21 is present in the genome of the patient as a structural chromosome aberration. The Cu/ZnSOD gene is localized on chromosome 21, closely associated with the gene complex responsible for the phenotype of Down's Syndrome. A gene-dosage effect for Cu/ZnSOD in Down's Syndrome providing a diagnostic marker for this syndrome has been described.
a) Patients with Down's Syndrome have significantly elevated serum and urine levels of Cu/ZnSOD.
b) Prenatal diagnosis of Down's Syndrome: Cu/ZnSOD levels are quantitated from erythrocytes of fetal umbilical vein blood and related to the number of cells, the content of haemoglobin and to the haematocrit. In case of Trisomy 21 the significantly elevated levels of Cu/ZnSOD are determined.
| Reactivity | Human |
|---|---|
| Sample Volume | 10 uL (1:20 prediluted) |
| Suitable Sample Types | cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine, fetal umbilical vein blood |
| Sensitivity | 0.04 ng/mL |
| Standard Curve Range | 0.08 - 5.0 ng/mL |
| Components | Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Cu/ZnSOD HRP-Conjugate anti-human Cu/ZnSOD monoclonal antibody Human Cu/ZnSOD Standard, 5 ng/mL Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA) Phosphate Buffered Saline Concentrate (PBS) 20x Wash Buffer Concentrate 20x (PBS with 1% Tween 20) Substrate Solution (tetramethyl-benzidine) Stop Solution (1M Phosphoric acid) Adhesive Films |
| Reported Applications | ELISA |
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Cu/ZnSOD ELISA kit参考文献
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Stewart,Victoria C.; Stone,Rebecca; Gegg,Matthew E.; Sharpe,Martyn A.; Hurst,Roger D.; Clark,John B.; Heales,Simon J.R.. Preservation of extracellular glutathione by an astrocyte derived factor with properties comparable to extracellular superoxide dismutase. Journal of Neurochemistry 2002;83:984-991. (Link)
Mangiagalli,Anna; Samuele,Alberta; Armentero,Marie Therese; Bazzini,Eleonora; Nappi,Giuseppe; Blandini,Fabio. Effects of homocysteine on apoptosis-related proteins and anti-oxidant systems in isolated human lymphocytes. European Journal of Biochemistry 2004;271:1671-1676. (Link)
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Pawlak,K.; Brzosko,S.; Mysliwiec,M.; Pawlak,D.. Kynurenine, quinolinic acid-The new factors linked to carotid atherosclerosis in patients with end-stage renal disease. Atherosclerosis 2009;204:561-566. (Link)
Pawlak,K.; Domaniewski,T.; Mysliwiec,M.; Pawlak,D.. The kynurenines are associated with oxidative stress, inflammation and the prevalence of cardiovascular disease in... Atherosclerosis 2009;204:309-314. (Link)
Ceroni,M.; Malaspina,A.; Poloni,T.E.; Alimonti,D.; Rognoni,F.; Habgood,J.; Imbesi,F.; Antonelli,P.; Alfonsi,E.; Curti,D.; deBelleroche,J.. Clustering of ALS patients in central Italy due to the occurrence of the L84F SOD1 gene mutation. Neurology 1999;53:1064(Link)
Pawlak,Krystyna; Mysliwiec,Michal; Pawlak,Dariusz. Oxidative stress, phosphate and creatinine levels are independently associated with vascular endothelial growth factor levels in patients with chronic renal failure. Cytokine 2008;43:(Link)
BLANDINI,F.; MANGIAGALLI,A.; COSENTINO,M.; MARINO,F.; SAMUELE,A.; RASINI,E.; FANCELLU,R.; MARTIGNONI,E.; RIBOLDAZZI,G.; CALANDRELLA,D.; FRIGO,G.M.; NAPPI,G.. Peripheral Markers of Apoptosis in Parkinson's Disease. The Effect of Dopaminergic Drugs. Annals of the New York Academy of Sciences 2003;1010:675-678. (Link)
Lucotti,Pietro; Setola,Emanuela; Monti,Lucilla D.; Galluccio,Elena; Costa,Sabrina; Sandoli,Emilia P.; Fermo,Isabella; Rabaiotti,Giovanni; Gatti,Roberto; Piatti,PierMarco. Beneficial effects of a long-term oral L-arginine treatment added to a hypocaloric diet and exercise training program in obese, insulin-resistant type 2 diabetic patients. Endocrinology and Metabolism 2006;291:E906-E912. (Link)References: Hartz,J.W.; Deutsch,H.F.. Subunit structure of human superoxide dismutase. J.Biol.Chem. 1972;247:7043-7050. (Link)
Shull,S.; Heintz,N.H.; Periasamy,M.; Manohar,M.; Janssen,Y.M.; Marsh,J.P.; Mossman,B.T.. Differential regulation of antioxidant enzymes in response to oxidants. J.Biol.Chem. 1991;266:24398-24403. (Link)
Niwa,Y.; Iizawa,O.; Ishimoto,K.; Akamatsu,H.; Kanoh,T.. Age-dependent basal level and induction capacity of copper-zinc and manganese superoxide dismutase and other scavenging enzyme activities in leukocytes from young and elderly adults. Am.J.Pathol. 1993;143:312-320. (Link)
Suzuki,H.; Matsumori,A.; Matoba,Y.; Kyu,B.S.; Tanaka,A.; Fujita,J.; Sasayama,S.. Enhanced expression of superoxide dismutase messenger RNA in viral myocarditis. An SH-dependent reduction of its expression and myocardial injury. J.Clin.Invest 1993;91:2727-2733. (Link)
Jolly,S.R.; Kane,W.J.; Bailie,M.B.; Abrams,G.D.; Lucchesi,B.R.. Canine myocardial reperfusion injury. Its reduction by the combined administration of superoxide dismutase and catalase. Circ.Res. 1984;54:277-285. (Link)
Goebel,K.M.; Storck,U.. Effect of intra-articular orgotein versus a corticosteroid on rheumatoid arthritis of the knees. Am.J.Med. 1983;74:124-128. (Link)
Herouart,D.; Van,Montagu M.; Inze,D.. Redox-activated expression of the cytosolic copper/zinc superoxide dismutase gene in Nicotiana. Proc.Natl.Acad.Sci.U S A 1993;90:3108-3112. (Link)
Porstmann,T.; Wietschke,R.; Cobet,G.; Lorenz,K.; Grunow,R.; Jahn,S.; Bollmann,R.; Stamminger,G.; von,Baehr R.. Immunochemical quantification of Cu/Zn superoxide dismutase in prenatal diagnosis of Down's syndrome. Hum.Genet. 1990;85:362-366. (Link)
Barra,D.; Martini,F.; Bannister,J.V.; Schinina,M.E.; Rotilio,G.; Bannister,W.H.; Bossa,F.. The complete amino acid sequence of human Cu/Zn superoxide dismutase. FEBS Lett. 1980;120:53-56. (Link)
Porstmann,T.; Wietschke,R.; Schmechta,H.; Grunow,R.; Porstmann,B.; Bleiber,R.; Pergande,M.; Stachat,S.; von,Baehr R.. A rapid and sensitive enzyme immunoassay for Cu/Zn superoxide dismutase with polyclonal and monoclonal antibodies. Clin.Chim.Acta 1988;171:1-10. (Link)
Porstmann,T.; Wietschke,R.; Cobet,G.; Stamminger,G.; Bollmann,R.; Rogalski,V.; Pas,P.. Cu/Zn superoxide dismutase quantification from fetal erythrocytes--an efficient confirmatory test for Down's syndrome after maternal serum screening and sonographic investigations. Prenat.Diagn. 1991;11:295-303. (Link)
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