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总DNA甲基化极易定量检测试剂
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5-mC
总DNA甲基化极易定量检测试剂盒,ELISA比色法
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
研卉生物
48T
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit
5-mC DNA ELISA Kit
Global DNA Methylation ELISA
Direct ELISA
Indirect ELISA
Isolated ssDNA or dsDNA, 200 bps – full length
Isolated DNA (requires single strand conversion)
Urine; Isolated DNA (requires single strand conversion, nuclease digestion, and alkaline phosphatase treatment)
100 ng optimal
2 µg of DNA minimum per assay
0.05% of 5-mC per 100 ng DNA
Lowest point in standard curve is 5% of 5-mC
150 nM of 5-mC deoxynucleoside
Synthesized PC (methylated) and NC (unmethylated) oligos
PC and NC E. coli gDNA (require single strand conversion); PC methylated with CpG Methylase
5-mC deoxynucleoside standard
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