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Human TNF alpha(C008)

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  • ¥480 - 7200
  • SinoBio
  • 国产
  • C008
  • 2025年11月08日
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    • 保存条件

      蛋白表达区间:Val77-Leu233

    • 保质期

      See instructions

    • 英文名

      标签:Tag free

    • 库存

      表达系统:E.coli

    • 供应商

      上海经科化学科技有限公司

    • 规格

      10ug/50ug/500ug/1mg

    规格:10ug产品价格:¥480.0
    规格:50ug产品价格:¥960.0
    规格:500ug产品价格:¥5040.0
    规格:1mg产品价格:¥7200.0
    产品描述
    Recombinant Human Tumor Necrosis Factor Alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed.
     
    蛋白编号
    P01375
     
    产品别称
    Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
     
    分子量
    17.5 KDa
     
    表观分子量
    16 KDa, reducing conditions
     
    剂型描述
    Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 6.0.
     
    内毒素
    < 0.01 EU/µg as determined by LAL test.
     
    纯度-SDS-PAGE
    Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
     
    纯度-SEC-HPLC
    Greater than 95% as determined by SEC-HPLC. (QC verified)
     
    生物活性-Cell Based Assay
    Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/ml. (QC verified)
     
    复溶
    • Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
    • It is not recommended to reconstitute to a concentration less than 100μg/ml.
    • Dissolve the lyophilized protein in distilled water.
    • Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
     
    运输条件
    • The product is shipped at ambient temperature.
    • Upon receipt, store it immediately at the temperature listed below.
     
    保存条件
    • Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
    • Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
    • Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
     
    产品背景
    TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease.

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    图标文献和实验
    该产品被引用文献

    TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease.

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    文献支持
    Human TNF alpha(C008)
    ¥480 - 7200