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- 2025年11月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
蛋白表达区间:Gln26-Ala189
- 保质期：
See instructions
- 英文名：
标签:C-6His
- 库存：
表达系统:E.coli
- 供应商：
上海经科化学科技有限公司
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ￥720.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥2000.0 |
| 规格： | 500ug | 产品价格： | ￥8960.0 |
| 规格： | 1mg | 产品价格： | ￥12800.0 |
- Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
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文献和实验Stem cell factor (SCF), is the ligand for the receptor-type protein-tyrosine kinase KIT. It plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. It also promotes phosphorylation of PIK3R1, which is the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5.
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
R&D Systems 96孔高通量蛋白芯片（Proteme Profiler 96 ）试剂盒技术专辑
省时省力，避免重复多次免疫沉淀或Western Blot实验---在单一样本中可同时检测、筛选16种待测物目标蛋白分子。 快速——3.5小时被完成实验。 高通量，微量样本---使用标准的96微孔板形式。 可应用与目的蛋白的大规模筛选研究——每个试剂盒可检测96个样品，每个试剂盒可最多检测768个指标
免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，SCF 浓度与 OD 值成正比，可通过绘制标准曲线求出标本中SCF 浓度。 试剂盒组成 （ 2-8 ℃保存） 酶标 板 （Coated Wells ） 96 孔
技术资料