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CometAssay® Silver Staining Kit
Trevigen’s CometAssay® Silver Staining Kit is designed for the convenient silver staining of CometAssay® or single cell gel electrophoresis results. Recording of data generated by the CometAssay® and staining with fluorescent dyes requires epifluorescence microscopy and rapid analysis because of the generally short lifespan of the dye. Using the Silver Staining kit, permanent records are created and visualization using standard light microscopy is possible. The Silver Staining Kit was designed specifically for use with the CometSlides™ to minimize unwanted background and the amount of hazardous waste generated by silver nitrate.
Features:
Convenient format.
Optimized for CometSlide staining.
Reduced hazardous waste.
CometAssay® Silver Staining Kit 彗星®银染试剂盒Applications:
CometAssay®
DNA Damage detection
Cat# 4254-200-K CometAssay® Silver Staining Kit
Components: Part # Component Size
4254-200-01 20X Staining Reagent #1 1.2 ml
4254-200-02 20X Staining Reagent #2 1.2 ml
4254-200-03 20X Staining Reagent #3 1.2 ml
4254-200-04 2X Staining Reagent #4 1.2 g
4254-200-05 10X Fixation Additive 2.2 ml
Cat# 4251-050-K CometAssay® Silver
CometAssay® Silver Staining Kit 彗星®银染试剂盒Components: Part # Component Size
4250-050-01 CometAssay® Lysis Solution 2 x 500 ml
4250-050-02 CometAssay® LMAgarose 15 ml
4250-050-03 CometSlide (2 well slide) 25 each
4250-050-04 200 mM EDTA; pH 10 12.5 ml
4254-200-01 20X Staining Reagent #1 1.2 ml
4254-200-02 20X Staining Reagent #2 1.2 ml
4254-200-03 20X Staining Reagent #3 1.2 ml
4254-200-04 2X Staining Reagent #4 1.2 g
4254-200-05 10X Fixation Additive 2.2 ml
Storage: Store (cat# 4254-200-K) at room temperature.
* When reconstituted and stored at 4°C, component is stable for 3 months.
** All Components in the CometAssay® Silver Staining Kit (4254-200-K) are also included in the CometAssay® Silver (4251-050-K).
*** SYBR® Green I nucleic acid gel stain is a registered Trademark of Molecular Probes inc, Eugene, OR. For license terms, please click here.
CometAssay® Silver Staining Kit 彗星®银染试剂盒
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.You should wear clean,disposable gloves and never touch the gel bands directly.The following is a protocol used at the proteomics resource center for Silver staining.If you are using Silver staining kit from Sigma,Invitrogen,or other venders,please follow
%) acetic acid 25.0 ml (5%) dH2O 250.0 ml dH2O 425.0 ml Stop Solution (500 ml) Kit Reagents acetic acid 25.0 ml (5%) 1. Oxidizer: Dilute 1:10 with dH2O. dH2O 475.0 ml 2. Silver reagent: Dilute 1:10 with dH2O. 3. Developer: Dissolve 16 g per 500 ml
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