- 询价
- 美国eBioscince
- 美国/中国
- xy-2019
- 2025年07月12日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
EpiQuik Methylated DNA Immunoprecipitation Kit
- 库存：
1
- 供应商：
上海信裕
- 甲基化DNA免疫沉淀试剂盒详细信息：
-
Product Overview
The EpiQuik™ Methylated DNA Immunoprecipitation (MeDIP) Kit is a complete set of essential components which uses a proprietary and unique procedure/composition to enrich methylated DNA from cells. In the assay, a antibody specific to methyl cytosine is used to immunoprecipitate methylated genomic DNA. The immunoprecipitated methylated fractions can be then used for a standard DNA detection. The kit is suitable for combining the specificity of methylated DNA immunoprecipitation with qualitative and quantitative PCR, and southern blot as well as DNA microarray.
WHY CHOOSE THE EPIQUIK™ MeDIP KIT?- The fastest MeDIP method ever designed. The entire procedure can be completed within only 4 hours.
- Directly immunoprecipitate the methylated fractions of DNA from cell lysates.
- Highly efficient enrichment of methylated DNA: > 95%
- Strip microplate format allows manual or high throughput.
- Conveniently includes columns for DNA purification in order to save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Achieves very reliable and consistent assay conditions.
甲基化DNA免疫沉淀试剂盒Principle & Procedure
The EpiQuik™ Methylated DNA Immunoprecipitation (MeDIP) Kit contains all reagents required for carrying out a successful methylated DNA immunoprecipitation directly from mammalian cells. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA in the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
SCHEMATIC PROCEDURE: 
甲基化DNA免疫沉淀试剂盒Product Components
CP1 (wash buffer)
CP2 (antibody buffer)
CP3A (pre-lysis buffer)
CP3B (lysis buffer)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Normal mouse IgG (1 mg/ml)*
Anti-5-methylcytosine (1mg/ml)*
Proteinase K (10 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide
* Spin the solution down to the bottom before use.Frequently Asked Qs
What is the difference between the Methylamp Methylated DNA Capture Kit (#P-1015) and the EpiQuik Methylated DNA Immunoprecipitation Kit (#P-2019)?
The Methylamp Methylated DNA Capture Kit is for purified DNA as the starting materials while the EpiQuik Methylated DNA Immunoprecipitation Kit is for nuclear extract as the starting materials.
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一、 什么是DNA甲基化在甲基转移酶的催化下，DNA的CG两个核苷酸的胞嘧啶被选择性地添加甲基，形成5－甲基胞嘧啶，这常见于基因的5'-CG-3'序列。大多数脊椎动物基因组DNA都有少量的甲基化胞嘧啶，主要集中在基因5'端的非编码区，并成簇存在。甲基化位点可随DNA的复制而遗传，?因为DNA复制后，甲基化酶可将新合成的未甲基化的位点进行甲基化。DNA 甲基化是表观遗传学的重要部分，同组蛋白修饰相互作用，通过改变染色质结构，调控基因表达。在哺乳类细胞或人体细胞中，DNA 甲基化与细胞的增殖、衰老
2.2.10 DNA微阵列法 Yan等2001年[40]将以分子杂交为基础的微阵列技术应用于DNA甲基化检测中，这种方法是基于杂交的寡核苷酸微阵列，是一种在基因组中寻找新位点的方法。包括用于整个基因组范围内扫描的差异甲基化（DMH）杂交（Huang等1999年[41]）和用于检测某个位点的甲基化特异性微阵列MSO（Gitan等2002年[42]）。前者类似于mRNA表达谱或cDNA微阵列，是CpG岛微阵列，后者类似于寡核苷酸微阵列，是针对CpG二核苷酸位点的甲基化特异性寡核苷酸微阵列[26
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