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- 上海钰博
- 进口/国产
- YB525Hu02
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Plasminogen Activator, Tissue (tPA)
- 库存：
10000
- 供应商：
钰博生物
组织型纤溶酶原激活因子(tPA)重组蛋白
适用生物 Homo sapiens (Human，人)
组织型纤溶酶原激活因子(tPA)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 59.8kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybD202Mu01 染色质域解旋酶DNA结合蛋白4(CHD4)重组蛋白 Recombinant Chromodomain Helicase DNA Binding Protein 4 (CHD4) Mus musculus (Mouse)
ybD206Mu01 EB病毒诱导蛋白3(EBI3)重组蛋白 Recombinant Epstein Barr Virus Induced Protein 3 (EBI3) Mus musculus (Mouse)
ybD207Hu01 补体成分1q子成分A(C1qA)重组蛋白 Recombinant Complement Component 1, Q Subcomponent A (C1qA) Homo sapiens (Human)
ybD219Hu01 胸腺素β10(TMSβ10)重组蛋白 Recombinant Thymosin Beta 10 (TMSb10) Homo sapiens (Human)
ybD220Mu01 胸腺旁腺素(PTMS)重组蛋白 Recombinant Parathymosin (PTMS) Mus musculus (Mouse)
ybD221Hu01 前胸腺素α(PTMα)重组蛋白 Recombinant Prothymosin Alpha (PTMa) Homo sapiens (Human)
ybD223Hu01 单酰甘油脂肪酶(MGL)重组蛋白 Recombinant Lipase, Monoacylglycerol (MGL) Homo sapiens (Human)
ybD226Hu01 胃脂酶(LIPF)重组蛋白 Recombinant Lipase, Gastric (LIPF) Homo sapiens (Human)
ybD228Hu01 快肌肌钙蛋白C(TNNC2)重组蛋白 Recombinant Troponin C Type 2, Fast (TNNC2) Homo sapiens (Human)
ybD229Hu01 骨骼肌慢肌肌钙蛋白I(TNNI1)重组蛋白 Recombinant Troponin I Type 1, Slow Skeletal (TNNI1) Homo sapiens (Human)
ybD230Ra01 骨骼肌快肌肌钙蛋白I(TNNI2)重组蛋白 Recombinant Troponin I Type 2, Fast Skeletal (TNNI2) Rattus norvegicus (Rat)
ybD232Hu01 心肌肌钙蛋白T(TNNT2)重组蛋白 Recombinant Troponin T Type 2, Cardiac (TNNT2) Homo sapiens (Human)
ybD234Hu01 角蛋白6A(KRT6A)重组蛋白 Recombinant Keratin 6A (KRT6A) Homo sapiens (Human)
ybD236Hu01 白三烯A4水解酶(LTA4H)重组蛋白 Recombinant Leukotriene A4 Hydrolase (LTA4H) Homo sapiens (Human)
ybD251Hu01 线粒体铁蛋白(FTMT)重组蛋白 Recombinant Ferritin, Mitochondrial (FTMT) Homo sapiens (Human)
ybD252Hu01 饥饿素/肥胖抑制素前激素原(GHRL)重组蛋白 Recombinant Ghrelin/Obestatin Preprohormone (GHRL) Homo sapiens (Human)
ybD263Hu01 细胞周期素A1(CCNA1)重组蛋白 Recombinant Cyclin A1 (CCNA1) Homo sapiens (Human)
ybD264Hu01 细胞周期素B(CCNB)重组蛋白 Recombinant Cyclin B (CCNB) Homo sapiens (Human)
ybD265Hu01 细胞周期素B2(CCNB2)重组蛋白 Recombinant Cyclin B2 (CCNB2) Homo sapiens (Human)
适用生物 Homo sapiens (Human，人)
组织型纤溶酶原激活因子(tPA)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 59.8kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Plasminogen Activator, Tissue (tPA)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPA525Hu02 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 59.8kDa |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Ile311~Pro562 (Accession # P00750) with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- IKGGLFADIA SHPWQAAIFA KHRRSPGERF LCGGILISSC WILSAAHCFQ ERFPPHHLTV ILGRTYRVVP GEEEQKFEVE KYIVHKEFDD DTYDNDIALL QLKSDSSRCA QESSVVRTVC LPPADLQLPD WTECELSGYG KHEALSPFYS ERLKEAHVRL YPSSRCTSQH LLNRTVTDNM LCAGDTRSGG PQANLHDACQ GDSGGPLVCL NDGRMTLVGI ISWGLGCGQK DVPGVYTKVT NYLDWIRDNM RP
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- IKGGLFADIA SHPWQAAIFA KHRRSPGERF LCGGILISSC WILSAAHCFQ ERFPPHHLTV ILGRTYRVVP GEEEQKFEVE KYIVHKEFDD DTYDNDIALL QLKSDSSRCA QESSVVRTVC LPPADLQLPD WTECELSGYG KHEALSPFYS ERLKEAHVRL YPSSRCTSQH LLNRTVTDNM LCAGDTRSGG PQANLHDACQ GDSGGPLVCL NDGRMTLVGI ISWGLGCGQK DVPGVYTKVT NYLDWIRDNM RP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
组织型纤溶酶原激活因子(tPA)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
组织型纤溶酶原激活因子(tPA)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and eas
ybD198Hu01 死骨片1(SQSTM1)重组蛋白 Recombinant Sequestosome 1 (SQSTM1) Homo sapiens (Human)
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and eas
ybD202Mu01 染色质域解旋酶DNA结合蛋白4(CHD4)重组蛋白 Recombinant Chromodomain Helicase DNA Binding Protein 4 (CHD4) Mus musculus (Mouse)
ybD206Mu01 EB病毒诱导蛋白3(EBI3)重组蛋白 Recombinant Epstein Barr Virus Induced Protein 3 (EBI3) Mus musculus (Mouse)
ybD207Hu01 补体成分1q子成分A(C1qA)重组蛋白 Recombinant Complement Component 1, Q Subcomponent A (C1qA) Homo sapiens (Human)
ybD219Hu01 胸腺素β10(TMSβ10)重组蛋白 Recombinant Thymosin Beta 10 (TMSb10) Homo sapiens (Human)
ybD220Mu01 胸腺旁腺素(PTMS)重组蛋白 Recombinant Parathymosin (PTMS) Mus musculus (Mouse)
ybD221Hu01 前胸腺素α(PTMα)重组蛋白 Recombinant Prothymosin Alpha (PTMa) Homo sapiens (Human)
ybD223Hu01 单酰甘油脂肪酶(MGL)重组蛋白 Recombinant Lipase, Monoacylglycerol (MGL) Homo sapiens (Human)
ybD226Hu01 胃脂酶(LIPF)重组蛋白 Recombinant Lipase, Gastric (LIPF) Homo sapiens (Human)
ybD228Hu01 快肌肌钙蛋白C(TNNC2)重组蛋白 Recombinant Troponin C Type 2, Fast (TNNC2) Homo sapiens (Human)
ybD229Hu01 骨骼肌慢肌肌钙蛋白I(TNNI1)重组蛋白 Recombinant Troponin I Type 1, Slow Skeletal (TNNI1) Homo sapiens (Human)
ybD230Ra01 骨骼肌快肌肌钙蛋白I(TNNI2)重组蛋白 Recombinant Troponin I Type 2, Fast Skeletal (TNNI2) Rattus norvegicus (Rat)
ybD232Hu01 心肌肌钙蛋白T(TNNT2)重组蛋白 Recombinant Troponin T Type 2, Cardiac (TNNT2) Homo sapiens (Human)
ybD234Hu01 角蛋白6A(KRT6A)重组蛋白 Recombinant Keratin 6A (KRT6A) Homo sapiens (Human)
ybD236Hu01 白三烯A4水解酶(LTA4H)重组蛋白 Recombinant Leukotriene A4 Hydrolase (LTA4H) Homo sapiens (Human)
ybD251Hu01 线粒体铁蛋白(FTMT)重组蛋白 Recombinant Ferritin, Mitochondrial (FTMT) Homo sapiens (Human)
ybD252Hu01 饥饿素/肥胖抑制素前激素原(GHRL)重组蛋白 Recombinant Ghrelin/Obestatin Preprohormone (GHRL) Homo sapiens (Human)
ybD263Hu01 细胞周期素A1(CCNA1)重组蛋白 Recombinant Cyclin A1 (CCNA1) Homo sapiens (Human)
ybD264Hu01 细胞周期素B(CCNB)重组蛋白 Recombinant Cyclin B (CCNB) Homo sapiens (Human)
ybD265Hu01 细胞周期素B2(CCNB2)重组蛋白 Recombinant Cyclin B2 (CCNB2) Homo sapiens (Human)
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文献和实验相关实验
以达到纯化抗体IgG的目的。一般管中收集的IgG抗体用蛋白胶（SDS-PAGE电泳）进行检测，并判断IgG的纯度和浓度。图4 精达公司重组蛋白A纯化柱产品 图5为广州精达公司蛋白A层析柱纯化后所制备的蛋白胶（SDS-PAGE电泳胶）。图5中，经精达公司层析柱洗脱后，从第1管至第8管均有抗体的重链和轻链的带型。将上述有蛋白带型的管进行合并后测量抗体的浓度，广州精达公司蛋白A平均抗体IgG的载率为52mg/ml。 上述IgG载率在诊断试剂及成本中有着十分重要的意义，众所周知，目前
摘要: 目的 探讨脑微血管内皮细胞的体外培养方法并进行细胞超微结构研究及组织型纤溶酶原激活物(TPA ) 活性测定。 方法 取新生小鼠脑组织, 通过匀浆、过筛、胶原酶消化、差速粘附等技术对鼠脑微血管内皮细胞进行原代培养, 待细胞铺满瓶底时, 用01125%胰酶20102%EDTA 消化, 离心收集内皮细胞, 进行传代培养。原代、传代各取8 例, 吸取培养液用酶联免疫吸附试验测试TPA 活性。 结果 经Ð 因子相关抗原免疫组织化学鉴定、细胞超微结构观察, 证明培养的是血管内皮细胞。培养
；并且也反映在血栓形成的局部纤溶酶活性或浓度超过血浆2‰—抗纤溶酶活性或浓度。溶栓治疗是指用药物来活化纤维蛋白溶解系统。一般为投入一种纤溶酶原活化物如尿液酶、链激酶或组织型纤溶酶原活化物（tpA），使大量纤溶酶生成，从而加速已形成血栓的溶解。FDP或D-二聚体生成，则表明达到溶栓效果。 纤溶蛋白降解产物中，唯D-二聚体交联碎片可反映血栓形成后的溶栓活性。因此，理论上，D-二聚体的定量检测可定量反映药物的溶栓效果、及可用于诊断、筛选新形成的血栓。但是，到目前为止，商品的D-二聚体检测手段都尚存在一定
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组织型纤溶酶原激活因子(tPA)重组蛋白
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