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- 2025年07月15日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Platelet Derived Growth Factor BB (PDGFBB)
- 库存：
10000
- 供应商：
钰博生物
血小板衍生生长因子BB(PDGFBB)重组蛋白
适用生物 Mus musculus (Mouse，小鼠)
血小板衍生生长因子BB(PDGFBB)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 57.0kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybG627Hu01 剪切修复交叉互补修复缺陷偶联因子1(ERCC1)重组蛋白 Recombinant Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) Homo sapiens (Human)
ybG628Hu01 髓细胞触发受体2(TREM2)重组蛋白 Recombinant Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) Homo sapiens (Human)
ybG647Hu01 RNA结合基元蛋白38(RBM38)重组蛋白 Recombinant RNA Binding Motif Protein 38 (RBM38) Homo sapiens (Human)
ybG669Hu01 氯离子通道辅助蛋白1(CLCA1)重组蛋白 Recombinant Chloride Channel Accessory 1 (CLCA1) Homo sapiens (Human)
ybG677Hu01 视锥蛋白样蛋白1(VSNL1)重组蛋白 Recombinant Visinin Like Protein 1 (VSNL1) Homo sapiens (Human)
ybG685Hu01 肌钯蛋白(MYPN)重组蛋白 Recombinant Myopalladin (MYPN) Homo sapiens (Human)
ybG687Mu01 原肌球调节蛋白3(TMOD3)重组蛋白 Recombinant Tropomodulin 3 (TMOD3) Mus musculus (Mouse)
ybG689Hu01 原肌球调节蛋白1(TMOD1)重组蛋白 Recombinant Tropomodulin 1 (TMOD1) Homo sapiens (Human)
ybG720Hu01 G蛋白偶联受体37(GPR37)重组蛋白 Recombinant G Protein Coupled Receptor 37 (GPR37) Homo sapiens (Human)
ybG751Hu01 TNF受体关联因子6(TRAF6)重组蛋白 Recombinant TNF Receptor Associated Factor 6 (TRAF6) Homo sapiens (Human)
ybG769Hu01 乳腺癌抗雌激素药物耐药性基因1(BCAR1)重组蛋白 Recombinant Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) Homo sapiens (Human)
ybG777Hu01 RNA聚合酶转录终止因子Ⅰ(TTF1)重组蛋白 Recombinant Transcription Termination Factor, RNA polymerase I (TTF1) Homo sapiens (Human)
适用生物 Mus musculus (Mouse，小鼠)
血小板衍生生长因子BB(PDGFBB)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 57.0kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Platelet Derived Growth Factor BB (PDGFBB)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | RPA633Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 57.0kDa |
| Predicted isoelectric point | 8.6 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | Secreted |
| Residues | Glu21~Ala241 (Accession # P31240) with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-EGDPIPEELY EMLSDHSIRS FDDLQRLLHR DSVDEDGAEL DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT RSPGTSREQR AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG A
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-EGDPIPEELY EMLSDHSIRS FDDLQRLLHR DSVDEDGAEL DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT RSPGTSREQR AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG A
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
血小板衍生生长因子BB(PDGFBB)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
血小板衍生生长因子BB(PDGFBB)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
血小板衍生生长因子BB(PDGFBB)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybG610Hu01 芳基硫酸酯酶B(ARSB)重组蛋白 Recombinant Arylsulfatase B (ARSB) Homo sapiens (Human)
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybG627Hu01 剪切修复交叉互补修复缺陷偶联因子1(ERCC1)重组蛋白 Recombinant Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) Homo sapiens (Human)
ybG628Hu01 髓细胞触发受体2(TREM2)重组蛋白 Recombinant Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) Homo sapiens (Human)
ybG647Hu01 RNA结合基元蛋白38(RBM38)重组蛋白 Recombinant RNA Binding Motif Protein 38 (RBM38) Homo sapiens (Human)
ybG669Hu01 氯离子通道辅助蛋白1(CLCA1)重组蛋白 Recombinant Chloride Channel Accessory 1 (CLCA1) Homo sapiens (Human)
ybG677Hu01 视锥蛋白样蛋白1(VSNL1)重组蛋白 Recombinant Visinin Like Protein 1 (VSNL1) Homo sapiens (Human)
ybG685Hu01 肌钯蛋白(MYPN)重组蛋白 Recombinant Myopalladin (MYPN) Homo sapiens (Human)
ybG687Mu01 原肌球调节蛋白3(TMOD3)重组蛋白 Recombinant Tropomodulin 3 (TMOD3) Mus musculus (Mouse)
ybG689Hu01 原肌球调节蛋白1(TMOD1)重组蛋白 Recombinant Tropomodulin 1 (TMOD1) Homo sapiens (Human)
ybG720Hu01 G蛋白偶联受体37(GPR37)重组蛋白 Recombinant G Protein Coupled Receptor 37 (GPR37) Homo sapiens (Human)
ybG751Hu01 TNF受体关联因子6(TRAF6)重组蛋白 Recombinant TNF Receptor Associated Factor 6 (TRAF6) Homo sapiens (Human)
ybG769Hu01 乳腺癌抗雌激素药物耐药性基因1(BCAR1)重组蛋白 Recombinant Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) Homo sapiens (Human)
ybG777Hu01 RNA聚合酶转录终止因子Ⅰ(TTF1)重组蛋白 Recombinant Transcription Termination Factor, RNA polymerase I (TTF1) Homo sapiens (Human)
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文献和实验相关实验
牢记N端法则：当N端第二个残基是Leu、Arg、Lys、Phe、Tyr和Trp时，重组蛋白半衰期只有2分钟，而小侧链的氨基酸残基，如Ala，则可以提高蛋白稳定性。因此在设计载体时，要特别注意第二个密码子，避免上述残基的出现。处理高毒性蛋白：毒性极高的重组蛋白，其本底表达就会杀死原核宿主，质粒容易丢失，使其在大肠杆菌中很难得到高表达。采用可表达T7溶菌酶的宿主。T7溶菌酶是T7RNA聚合酶的抑制剂，采用含有T7溶菌酶质粒的宿主，如pLysS，可以降低重组蛋白在大肠杆菌快速生长期的本底
有差异，批次一致性没有化学成分明确的培养基好； 5）病毒来源的风险和病毒检测成本极高； 6）HPL 来源有限，我国法律对血液制品提取物，包括 HPL 严格控制，无法实现产业化； 因此，HPL 是一个选择，但是仍然不完善，还有很多空间，因此科学家还在努力，寻找更好的办法。 普通 MSC 细胞培养基+重组蛋白因子混合物 是近年来不断优化的一个解决方案，已经无限接近化学成分完全明确了，但是还是有不少的缺点； 1）MSC 细胞生长需要各种蛋白因子，种类较多，20 种左右，这些蛋白和因子形成聚体或颗粒
/α亚族，主要趋化中性粒细胞，包括IL-8、黑素瘤细胞生长刺激活性(GRO/MGSA）、血小板因子-4（PF-4）、血小板碱性蛋白、蛋白水解来源的产物CTAP-III和β-thromboglobulin、炎症蛋白10（IP-10）、ENA-78；（2）C-C/β亚族，主要趋化单核细胞，包括巨噬细胞炎症蛋白1α（MIP-1α）、MIP-1β、RANTES、单核细胞趋化蛋白-1（MCP-1/MCAF）、MCP-2、MCP-3和I-309。 7.其它细胞因子，如表皮生长因子（EGF）、血小板衍生
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血小板衍生生长因子BB(PDGFBB)重组蛋白
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