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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1365 |
---|---|
产品名称 | Human IL-34 ELISA Kit |
产品描述 | For quantitative detection of human IL-34 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, N21-P242 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | IL34 |
基因全名 | interleukin 34 |
Uniprot ID | Q6ZMJ4 |
指标别名 | C16orf77|Chromosome 16 open reading frame 77|FPT025|IL 34|IL-34|IL34|IL34_HUMAN|Interleukin 34|Interleukin-34|Interleukin34|MGC34647 |
背景资料 | Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1365-CAP | 96-well plate precoated with anti- human IL-34 antibody | 1 |
EK1365-ST | Lyophilized recombinant human IL-34 standard | 10ng/tube×2 |
EK1365-DA | Biotinylated anti- human IL-34 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 93 | 548 | 1157 | 96 | 552 | 1148 |
Standard deviation | 5.3 | 33.42 | 67.1 | 5.85 | 35.32 | 73.47 |
CV(%) | 5.7% | 6.1% | 5.8% | 6.1% | 6.4% | 6.4% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.047 | 0.095 | 0.153 | 0.265 | 0.501 | 0.895 | 1.53 | 2.344 |
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