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- 保存条件:
低温
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现货
- 供应商:
上海善然生物科技有限公司
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
采用盐析法、离子交换法和凝胶过滤层析法制备。
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.1
Protocols & Applications
Select the right albumin for your application with the Albumin Selection Guide
性质
Related Categories Albumin, Albumins, Albumins and Transport Proteins, Albumins by Application, Bovine Serum Albumins,
Chromatographically Purified, Core Bioreagents, For Cell Culture, Life Science Reagents for Cell Culture, New Zealand Origin Bovine Serum Albumins, Research Essentials, 生化试剂, 细胞培养, 蛋白质及衍生物, 试剂和添加剂
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product line BioReagent
assay ≥95% (agarose gel electrophoresis)
form lyophilized powder
mol wt mol wt ~66 kDa
purified by chromatography
origin New Zealand origin
impurities IgG free
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文献和实验4.8 4.0 3.2 2.4 1.6 0.8 0 注:1%牛血清白蛋白,经凯氏定氮测定含纯蛋白为80%。
如何选择合适的牛血清白蛋白(BSA)? 由于牛血清白蛋白(BSA)具有多种产品样式,可满足不同种的应用。目前网上的信息大多模棱两可,有牛血清白蛋白,也有牛血清白蛋白v组分等,很多 供货商对其区别也是不清楚的;另外,市场上还提供无蛋白酶、无DNase及无RNase、无脂肪酸、微生物级、试剂级、诊断分析级等不同等BSA粉末,因 此如何选择合适的BSA产品是很重要的前提。一般来说,普通
由于牛血清白蛋白(BSA)具有多种产品样式,可满足不同种的应用。目前网上的信息大多模棱两可,有牛血清白蛋白,也有牛血清白蛋白v组分等,很多 供货商对其区别也是不清楚的;另外,市场上还提供无蛋白酶、无DNase及无RNase、无脂肪酸、微生物级、试剂级、诊断分析级等不同等BSA粉末,因 此如何选择合适的BSA产品是很重要的前提。一般来说,普通的BSA粉末或者第五组分适合于细胞和组织培养,也可以用于一些蛋白补充物;而其它无蛋白酶、 无DNase及无RNase、无脂肪酸等处理的BSA则适合于更多
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