人血浆纤维连接蛋白

Caution
Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
Preparation Note
Lyophilized from 0.05 M Tris buffered saline, pH 7.5
Application
Fibronectin from human plasma is used for the following applications:
• Used in cell seeding and neuronal differentiation (as one of the components in expansion medium for the re-suspension of CBSCs (Cord blood stem cells) and is also used in differentiation medium)[2]
• Used in cell culture (the culture chamber was coated with fibronectin for the expression of HEK293 cells)[5]
• Used in murine embroyonic stem cell line (CCE) culture and induction of endothelial cell differentiation (for the induction of endothelial cell differentiation, cultured cells were pre-coated with fibronectin)[6]
• In-vitro fibronectin (FN) pulldown assays[4]
• Protein specificity[8]
• Immunofluorescence (used in staining of cell layers for membrane type-1 matrix metalloproteinase (MT1-MMP) for coating of slides)[9]
• Extracellular matrix binding assay[1]
• Used in cell migration study[7]
Biochem/physiol Actions
Fibronectin (FN) plays an essential role in multiple biological functions such as, angiogenesis, cell migration and differentiation and wound healing. Studies in Chinese Han population show that mutations in this gene might be linked with susceptibility to knee osteoarthritis (OA).[10] Splice variant extra domain-B (EDB) of FN is a hallmark of tumor angiogenesis, and with increased matrix stiffness there is an increase in the production of this isoform by endothelial cells. In colorectal cancer (CRC), EDA is linked with metastasis, vasculogenesis and tumorigenesis and promotes epithelial-mesenchymal transition.[3]
General description
Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.[10]