Sigma货号E2129-100g伊文思蓝Evans Bule上海睿安生物13611631389
说明
一般描述
伊文思蓝(EB)C.I.23860是一种合成双偶氮染料,也称为T-1824和直接蓝53。水溶性较高的EB染料对血清白蛋白具有很强的亲和力,是一种高分子量蛋白质示踪剂。
应用
伊文思蓝是一种著名的重要染色剂,适用于组织学和荧光显微镜。伊文思蓝已用于检查细胞活力和研究血脑屏障(BBB)的渗透性。其他应用包括展示特定的垂体细胞类型、中性粒细胞质膜的选择性染色以及在荧光显微镜中作为一种猝灭剂抑制不必要的自体荧光。
生化/生理作用
伊文思蓝是一种非渗透染料。在质膜存在损伤时,染料可进入细胞质和核中并将它们染成蓝色。伊文思蓝可用于检查细胞的活力。它还可用于研究BBB(血脑屏障)的通透性。染料与白蛋白结合,从而表明BBB已分解为蛋白。
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