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- 2025年08月07日
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- 供应商：
上海善然生物科技有限公司
产品名称
General description
Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.
Other Notes
Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 541, Number of Clones: 2864
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
法律信息
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
性质
| Related Categories | MISSION shRNA, MISSION shRNA Gene Family Sets, 分子生物学, 功能基因组和RNAi |
| storage temp. | −20°C |
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文献和实验DNA Shuffling and Family Shuffling for In Vitro Gene Evolution
genes, instead of a set of mutant genes derived from a single gene, this technique is called “family shuffling” Fig. 2 ). Family shuffling utilizes naturally occurring nucleotide substitutions among family genes as the driving force
Family Shuffling with Single-Stranded DNA
Family shuffling, which generates chimeric progeny genes by recombining a set of naturally occurring homologous genes, is an extremely powerful approach for in vitro protein evolution. In comparison with other in vitro protein evolution methods
resource for gene analysis and discovery. 2004年3月30日，Open Biosystems公司正式宣布Expression Arrest? Human & Mouse Short Hairpin RNA (shRNA) Libraries对外发售。该Expression Arrest? shRNA Libraries构建者们设计、合成、测序并克隆了上千个小分子发夹RNA，研究者只需在网上数据库中选择特定的shRNA克隆就可在近期内得到用于RNAi
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