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发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin).
Mouse
96T
货号 | EK0717 |
---|---|
产品名称 | Mouse CTLA4 ELISA Kit |
产品描述 | For quantitative detection of mouse CTLA4 in cell culture supernates, serum and plasma(heparin). |
免疫原 | NSO, A37-F162 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2ng/ml→2000pg/ml |
基因名称 | CTLA4 |
基因全名 | cytotoxic T-lymphocyte-associated protein 4 |
Uniprot ID | P09793 |
指标别名 | Activation B7-1 antigen|B7|BB1|CD80|CD80_HUMAN|CTLA-4 counter-receptor B7.1|T-lymphocyte activation antigen CD80 |
背景资料 | CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152(Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0717-CAP | 96-well plate precoated with anti- mouse CTLA4 antibody | 1 |
EK0717-ST | Lyophilized recombinant mouse CTLA4 standard | 10ng/tube×2 |
EK0717-DA | Biotinylated anti- mouse CTLA4 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 312 | 1627 | 4692 | 332 | 1618 | 5074 |
Standard deviation | 17.47 | 69.96 | 304.98 | 20.25 | 77.66 | 334.88 |
CV(%) | 5.6% | 4.3% | 6.5% | 6.1% | 4.8% | 6.6% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.004 | 0.149 | 0.320 | 0.556 | 0.962 | 1.453 | 1.922 | 2.213 |
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