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发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA, citrate).
Mouse
96T
货号 | EK2070 |
---|---|
产品名称 | Mouse GFRA1 ELISA Kit |
产品描述 | For quantitative detection of mouse GFRA1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, D25-S430 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | GFRA1 |
基因全名 | glial cell line derived neurotrophic factor family receptor alpha 1 |
Uniprot ID | GFRA1: P97785 |
指标别名 | GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; TGF-beta-related neurotrophic factor receptor 1; Gfra1; Gdnfra; Trnr1 |
背景资料 | GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to 19 D2; 19 54.18 cM. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.GDNF family receptor alpha 1 has been shown to interact with GDNF and RET proto-oncogene. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2070-CAP | 96-well plate precoated with anti- mouse GFRA1 antibody | 1 |
EK2070-ST | Lyophilized recombinant mouse GFRA1 standard | 10ng/tube×2 |
EK2070-DA | Biotinylated anti- mouse GFRA1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 17 | 89 | 450 | 23 | 107 | 462 |
Standard deviation | 0.68 | 4.18 | 23.40 | 1.17 | 6.31 | 32.80 |
CV(%) | 4.0% | 4.7% | 5.2% | 5.1% | 5.9% | 7.1% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.039 | 0.130 | 0.242 | 0.507 | 0.824 | 1.311 | 1.776 | 2.194 |
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