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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0424 |
---|---|
产品名称 | Human IL-13 ELISA Kit |
产品描述 | For quantitative detection of human IL-13 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, G21-N132 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | IL13 |
基因全名 | interleukin 13 |
Uniprot ID | P35225 |
指标别名 | Allergic rhinitis|ALRH|BHR 1|BHR1|Bronchial hyperresponsiveness 1 (bronchial asthma)|IL 13|IL-13|Il13|IL13_HUMAN|Interleukin 13|Interleukin-13|Interleukin13|MGC116786MGC116788|MGC116789|NC30|P600 |
背景资料 | Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells which is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4R alpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0424-CAP | 96-well plate precoated with anti- human IL-13 antibody | 1 |
EK0424-ST | Lyophilized recombinant human IL-13 standard | 10ng/tube×2 |
EK0424-DA | Biotinylated anti- human IL-13 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 34 | 190 | 438 | 32 | 176 | 440 |
Standard deviation | 1.93 | 10.64 | 24.09 | 2.36 | 10.56 | 29.92 |
CV(%) | 5.7% | 5.6% | 5.5% | 7.4% | 6% | 6.8% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.016 | 0.053 | 0.099 | 0.195 | 0.392 | 0.865 | 1.874 | 2.707 |
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