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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0827 |
---|---|
产品名称 | Human RAGE ELISA Kit |
产品描述 | For quantitative detection of human Rage in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Q24-A344 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 78pg/ml-5000pg/ml |
基因名称 | AGER |
基因全名 | advanced glycosylation end product-specific receptor |
Uniprot ID | Q15109 |
指标别名 | Advanced glycosylation end product specific receptor|Advanced glycosylation end product-specific receptor|Ager|DAMA 358M23.4|LE 9211 A antigen |MAPK MAK MRK overlapping kinase |MGC22357|MOK|OTTHUMP00000174885|RAGE 1|RAGE isoform NtRAGE-delta|RAGE isoform sRAGE-delta|RAGE_HUMAN|RAGE1|Receptor for advanced glycation end products|receptor for advanced glycation end-products variant 20|Receptor for advanced glycosylation end products|Renal tumor antigen |Renal tumor antigen 1 |
背景资料 | RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B(NF-kappaB). |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0827-CAP | 96-well plate precoated with anti- human Rage antibody | 1 |
EK0827-ST | Lyophilized recombinant human Rage standard | 10ng/tube×2 |
EK0827-DA | Biotinylated anti- human Rage antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 154 | 623 | 3076 | 159 | 595 | 3344 |
Standard deviation | 10.16 | 27.41 | 221.47 | 10.65 | 32.72 | 250.8 |
CV(%) | 6.6% | 4.4% | 7.2% | 6.7% | 5.5% | 7.5% |
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.075 | 0.166 | 0.291 | 0.391 | 0.875 | 1.375 | 1.867 | 2.153 |
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