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上海善然生物科技有限公司
Frequently Asked QuestionsLive Chat andFrequently Asked Questions are available for this Product.Biochem/physiol ActionsHeparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization.2,3,4It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.5Other NotesEnzyme Commission Numbers: 4.2.2.7 Hep I and 4.2.2.8 Hep III包装Sold on the basis of Heparinase I unitsUnit DefinitionOne unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C usingHeparin, Sodium as substrate for heparinase I.
One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidneyHeparan, Sulfate as substrate for heparinase III.ApplicationHeparinase I and III may be used for the study of heparin production during fermentation and specific activity of heparinise.1General descriptionHeparinase is an inducible, non-extracellular heparin-degrading enzyme. Three types of heparinises areproduced byFlavobacterium heparinumand contains specific sequences of heparin.6
性质
Related Categories4.2.x.x C-O bonds,4.x.x.x Lyases,Application Index,Carbohydrate Hydrolysis,Carbohydrate hydrolysis & PTM analysis,
Carbohydrate-active Enzymes,Enzyme Class Index,Glycosaminoglycan (GAG) Degrading Enzymes,分子生物学,生化试剂,糖生物学,翻译后修饰,蛋白质组学,酶、抑制剂和底物更多...activity ≥200 units proteinshipped in dry icestorage temp. −20°C
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文献和实验菌体未被有效裂解。解决方法:注意将细菌悬浮充分。 (3)加 Solution III 后中和不充分。解决方法:如果细菌用量较多,请注意多翻转几次直至中和后的沉淀呈松散的豆腐花状(也可以稍用力混合直至沉淀呈松散的豆腐花状)。 (4)Solution II 出现沉淀。解决方法:如果实验室内温度低于 15℃,使用试剂盒前请注意观察 Solution II 中是否出现沉淀。如果出现沉淀,请于 37℃ 水浴溶解沉淀后再使用。 (5)Solution II 长时间暴露于空气中被 CO2 中和,导致菌体未能
核蛋白提取 实验准备: 1 4℃离心机 2 根据标本数计算所需I,II,III,IV总体积,取出液体,并加入蛋白酶抑制剂,(III由加蛋白酶抑制剂I与加蛋白酶抑制剂II混合物) 实验操作: 1 收集细胞。10ml 对照细胞(1-2*105/ml )培养48h后,15ml 离心管收集,300g(1370 rpm)* 5min。弃上清,加1ml 1*PBS轻轻涡旋混匀后转移至1.5ml EP管。 2 4℃,300g * 5min,弃上清。 3 重悬
bp) 双链RNA ,从而验证RNaseIII 的消化能力,结果表明,1个单位的RnaseIII 在37度1小时可以将1mg 的双链RNA 降解为30bp以下,主要是1215bp 的siRNA s 混合物。用其他基因的双链RNA ,同样证实RNaseIII 能够消化各种双链RNA 序列。另外用Cyclophillin, c-myc, Map Kinase 9, PKC-alpha, Raf-1, Nautilus, 和 h-ras 等基因的双链RNA 作为 RNase III 底物,也可以得到同样
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