- ¥80
- 上海钰博
- 进口/国产
- YB903Ra01
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Deiodinase, Iodothyronine, Type II (DIO2)
- 库存：
10000
- 供应商：
钰博生物
碘甲腺原氨酸脱碘酶Ⅱ(DIO2)重组蛋白
适用生物 Rattus norvegicus (Rat，大鼠)
碘甲腺原氨酸脱碘酶Ⅱ(DIO2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 72.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
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yb-5861R ADAMTSL1整合素样金属蛋白酶与凝血酶样1蛋白抗体 浓度1mg/ml
yb-5862R ADAMTSL2整合素样金属蛋白酶与凝血酶样2蛋白抗体 浓度1mg/ml
yb-5863R ADAMTSL3整合素样金属蛋白酶与凝血酶样3蛋白抗体 浓度1mg/ml
yb-5852R ADAM20去整合素样金属蛋白酶20抗体 浓度1mg/ml
yb-5853R ADAM23去整合素样金属蛋白酶23抗体 浓度1mg/ml
yb-5854R ADAM28去整合素样金属蛋白酶28抗体 浓度1mg/ml
适用生物 Rattus norvegicus (Rat，大鼠)
碘甲腺原氨酸脱碘酶Ⅱ(DIO2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 72.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Deiodinase, Iodothyronine, Type II (DIO2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | RPC903Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 72.4kDa |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | Ser30~Phe259 (Accession # P70551) with two N-terminal Tags, His-tag and MBP-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and MBP-tag, its sequence is listed below.
MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGSEF- S VILLKHVALL LSRSKSTRGE WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE VRSWLEKNF
MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGSEF- S VILLKHVALL LSRSKSTRGE WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE VRSWLEKNF
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
碘甲腺原氨酸脱碘酶Ⅱ(DIO2)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
碘甲腺原氨酸脱碘酶Ⅱ(DIO2)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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yb-1562R Annexin A1膜粘连蛋白A1抗体 浓度1mg/ml
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yb-1632R APPL1衔接因子蛋白含pH域蛋白1抗体 浓度1mg/ml
yb-2506R AQP7水通道蛋白-7抗体 浓度1mg/ml
yb-2532R ALT1/Alanine Transaminase谷丙氨酸氨基转移酶1抗体 浓度1mg/ml
yb-2936R ARHG7p21活化蛋白激酶ARHG7抗体 浓度1mg/ml
yb-6465R phospho-APE(Ser1417)磷酸化肌动蛋白结合蛋白Girdin抗体 浓度1mg/ml
yb-2640R ADO2-氨基乙硫醇双加氧酶抗体 浓度1mg/ml
yb-2097R Angiotensin II Type 1 Receptor血管紧张素Ⅱ-1型受体抗体 浓度1mg/ml
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yb-5958R AF 4 protein原癌基因AF4蛋白抗体 浓度1mg/ml
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yb-5853R ADAM23去整合素样金属蛋白酶23抗体 浓度1mg/ml
yb-5854R ADAM28去整合素样金属蛋白酶28抗体 浓度1mg/ml
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文献和实验相关实验
冰冻，室温融解，反复几次，由于细胞内冰粒形成和剩余细胞液的盐浓度增高引起溶胀，使细胞结构破碎。 5.化学处理法：有些动物细胞，例如肿瘤细胞可采用十二烷基磺酸钠（SDS）、去氧胆酸钠等细胞膜破坏，细菌细胞壁较厚，可采用溶菌酶处理效果更好，我用的浓度一般为1mg/ml。 无论用哪一种方法破碎组织细胞，都会使细胞内蛋白质或核酸水解酶释放到溶液中，使大分子生物降解，导致天然物质量的减少，加入二异丙基氟磷酸（DFP）可以抑制或减慢自溶作用；加入碘乙酸可以抑制那些活性中心需要有疏基的蛋白水解酶的活性，加入
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碘甲腺原氨酸脱碘酶Ⅱ(DIO2)重组蛋白
¥80
