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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
5mg
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文献和实验TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
with tissue adherence on slides during the hybridization procedure. Immerse tissue in sterile 15% sucrose/1xPBS (recipe follows) 3 hrs. to overnight at 4°C. Embed tissue in O.C.T. (Baxter No. M7148-4), M1 (Lipshaw) or any other convenient embedding
TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
for up to two weeks. 15% Sucrose in PBS: 500ml sterile PBS 75g "RNase free" sucrose Mix above and filter sterilize with a disposable Nalgene filtration unit type S(0.45 micron). Store at 4°C. USE OF FISHERBRAND SUPERFROST
10. (take muscle F-actin; not supernatant) homogenize in 300 ml buffer G at +4 oC; dialyze suspension against buffer G for 48 h; centrifuge at 100,000 x g for 2 h 11. (take muscle G-actin; not pellet) chromatograph on sephacryl S-300 column
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