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文献和实验Fungal Midi DNA Kit Protocol for Fresh/Frozen Specimens
实验试剂 1. Equilibrate sterile dH2 O water or DNA Elution Buffer at 65°C. 2. Isopropyl alcohol (isopropanol) 3. Absolute (96%-100%) ethanol 4. RNase A stock solution at 20 mg/mL 实验设备
Fastfilter Plasmid Midi Kit Spin Protocol
with Lysate Clearance Filter Syringe. Note: Step 10 to 16 should be performed in swinging-bucket rotor for maximal plasmid DNA yields. All of centrifugation steps must be carried out at room temperature. Purify Plasmid DNA with HiBindTM DNA Midi
E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
tubes or microplates 实验步骤 1. Add 1-100 ul blood sample to a nuclease-free microcentrifuge tube. Bring the volume up to 100 ul with Elution Buffer provided with this kit. 2. Add 100 ul Buffer MSL and 10 ul Proteinase K
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