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文献和实验Fungal Midi DNA Kit Protocol for Fresh/Frozen Specimens
实验试剂 1. Equilibrate sterile dH2 O water or DNA Elution Buffer at 65°C. 2. Isopropyl alcohol (isopropanol) 3. Absolute (96%-100%) ethanol 4. RNase A stock solution at 20 mg/mL 实验设备
Fastfilter Plasmid Midi Kit Spin Protocol
with Lysate Clearance Filter Syringe. Note: Step 10 to 16 should be performed in swinging-bucket rotor for maximal plasmid DNA yields. All of centrifugation steps must be carried out at room temperature. Purify Plasmid DNA with HiBindTM DNA Midi
DNA Purification from Blood or Body Fluids
QIAGEN Protease (or proteinase K) and 400 µl Buffer AL. If sample volumes larger than 400 µl are required, use of QIAamp DNA Blood Midi or Maxi Kits is recommended; these can process up to 2 ml or up to 10 ml of sample, respectively. Note
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