磷酸酶抑制剂Cocktail

For Phosphorylation Protection, more inhibitors mean better results. So we provide Two Tubes with 8 inhibitors.
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. For decades, researchers have used easily-accessible phosphate mimetics (tube A) to prevent the loss of phosphorylation on biomolecules in vitro. In recent years however, a number of naturally derived agents (tube B) have been discovered as potent inhibitors of phosphatases, thus increasing the available protection spectrum.

Contents

Tube #	Ingredient	100x Conc.	Target
A (Aqueous)	Sodium Fluoride	100 mM	Acid phosphatases
	Sodium Orthovanadate	100 mM	Alkaline phosphatases, PTPs, ATPases
	Sodium Tartrate	400 mM	Acid phosphatases
	Sodium Molybdate	115 mM	Acid and phosphoprotein phosphatases
	Imidazole	200 mM	Alkaline phosphatases
B (DMSO)	(-)-p-Bromotetramisole oxalate	2.5 mM	Alkaline phosphatases
	Cantharidin	500 μM	Ser/Thr phosphatases
	Microcystin LR, Microcystis aeruginosa	500 nM	PP1 and PP2A

Works well in common detergents such as 1% SDS, Triton, and NP-40
DTT, EDTA and EGTA may reduce the protection effects of Sodium Orthovanadate

储存条件

The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.

实验方法

Application Fields	Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Experiment Protocol	Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Note:
For optimum protection of phosphorylated proteins and lipids, we recommend simultaneous use of BOTH Tubes A and B. Because the inhibitor cocktails target different families of phosphatases, synergistic stabilization effects are observed with dual-treatment.

The Phosphatase Inhibitor Cocktail does not contain protease inhibitors. Therefore,we recommend that a protease inhibitor cocktail is added beforehand. Protease inhibitor cocktails are frequently used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.