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- YB537Hu01
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Enolase, Neuron Specific (NSE)
- 库存：
10000
- 供应商：
钰博生物
- 规格：
50ug
神经元特异性烯醇化酶(NSE)重组蛋白
适用生物 Homo sapiens (Human，人)
神经元特异性烯醇化酶(NSE)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 48.8kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
yb-1227R Ack1醋酸激酶1抗体 浓度1mg/ml
yb-0443R ACTH (1-39)促肾上腺皮质激素(1-39)抗体 浓度1mg/ml
yb-0442R ACTH (18-39)促肾上腺皮质激素ACTH(18-39)抗体 浓度1mg/ml
yb-0004R ACTH (7-23)促肾上腺皮质激素ACTH (7-23)抗体 浓度1mg/ml
yb-0039R ADNP活性依赖的神经保护肽抗体 浓度1mg/ml
yb-6852R Alpha 1 Acid Glycoproteinα1酸性糖蛋白1/类粘蛋白1抗体 浓度1mg/ml
yb-7565R Alpha 1 acid glycoprotein 2α1酸性糖蛋白2抗体（类粘蛋白2） 浓度1mg/ml
yb-4610R phospho-AQP2(Ser264+261)磷酸化水通道蛋白2抗体 浓度1mg/ml
yb-1907R ATTY细胞酪氨酸转氨酶抗体 浓度1mg/ml
yb-1930R Vasopressin抗利尿激素/血管升压素/加压素/血管加压素抗体 浓度1mg/ml
yb-1960R ABCF1ATP结合盒蛋白家族GCN20F家族1抗体 浓度1mg/ml
yb-2405R AKR1B1醛糖还原酶抗体 浓度1mg/ml
yb-0945R Serum albumin人血清白蛋白抗体 浓度1mg/ml
yb-0935R Anthrax炭疽菌抗体（采用活疫苗制备） 浓度1mg/ml
yb-1531R ATF4活化转录因子4抗体 浓度1mg/ml
yb-0299R Adenylate Kinase 1腺苷酸激酶-1抗体 浓度1mg/ml
yb-1571R F-Actin纤维状肌动蛋白抗体 浓度1mg/ml
yb-1176R Agrin聚集蛋白抗体 浓度1mg/ml
yb-0037M AIF调亡诱导因子抗体 浓度1mg/ml
yb-0037R AIF调亡诱导因子抗体 浓度1mg/ml
yb-0097R ALK间变型淋巴瘤激酶抗体 浓度1mg/ml
yb-0840R AMACRα-甲基酰基辅酶A消旋酶抗体 浓度1mg/ml
yb-1562R Annexin A1膜粘连蛋白A1抗体 浓度1mg/ml
yb-3602R ATG1/ULK1自噬相关蛋白1抗体 浓度1mg/ml
适用生物 Homo sapiens (Human，人)
神经元特异性烯醇化酶(NSE)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 48.8kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Enolase, Neuron Specific (NSE)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPA537Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 48.8kDa |
| Predicted isoelectric point | 5.1 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | Cytoplasm. Cell membrane |
| Residues | Met1~Leu434 (Accession # P09104) with N-terminal His-Tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
MGHHHHHHSGSEF-MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
USAGE
神经元特异性烯醇化酶(NSE)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
神经元特异性烯醇化酶(NSE)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-0120R AcinusAcinus抗体 浓度1mg/ml
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
神经元特异性烯醇化酶(NSE)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-1227R Ack1醋酸激酶1抗体 浓度1mg/ml
yb-0443R ACTH (1-39)促肾上腺皮质激素(1-39)抗体 浓度1mg/ml
yb-0442R ACTH (18-39)促肾上腺皮质激素ACTH(18-39)抗体 浓度1mg/ml
yb-0004R ACTH (7-23)促肾上腺皮质激素ACTH (7-23)抗体 浓度1mg/ml
yb-0039R ADNP活性依赖的神经保护肽抗体 浓度1mg/ml
yb-6852R Alpha 1 Acid Glycoproteinα1酸性糖蛋白1/类粘蛋白1抗体 浓度1mg/ml
yb-7565R Alpha 1 acid glycoprotein 2α1酸性糖蛋白2抗体（类粘蛋白2） 浓度1mg/ml
yb-4610R phospho-AQP2(Ser264+261)磷酸化水通道蛋白2抗体 浓度1mg/ml
yb-1907R ATTY细胞酪氨酸转氨酶抗体 浓度1mg/ml
yb-1930R Vasopressin抗利尿激素/血管升压素/加压素/血管加压素抗体 浓度1mg/ml
yb-1960R ABCF1ATP结合盒蛋白家族GCN20F家族1抗体 浓度1mg/ml
yb-2405R AKR1B1醛糖还原酶抗体 浓度1mg/ml
yb-0945R Serum albumin人血清白蛋白抗体 浓度1mg/ml
yb-0935R Anthrax炭疽菌抗体（采用活疫苗制备） 浓度1mg/ml
yb-1531R ATF4活化转录因子4抗体 浓度1mg/ml
yb-0299R Adenylate Kinase 1腺苷酸激酶-1抗体 浓度1mg/ml
yb-1571R F-Actin纤维状肌动蛋白抗体 浓度1mg/ml
yb-1176R Agrin聚集蛋白抗体 浓度1mg/ml
yb-0037M AIF调亡诱导因子抗体 浓度1mg/ml
yb-0037R AIF调亡诱导因子抗体 浓度1mg/ml
yb-0097R ALK间变型淋巴瘤激酶抗体 浓度1mg/ml
yb-0840R AMACRα-甲基酰基辅酶A消旋酶抗体 浓度1mg/ml
yb-1562R Annexin A1膜粘连蛋白A1抗体 浓度1mg/ml
yb-3602R ATG1/ULK1自噬相关蛋白1抗体 浓度1mg/ml
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文献和实验相关实验
近年来，肺癌的肿瘤标志物（tumor marker，TM）研究十分活跃。肺癌常用的肿瘤标志物很多，根据美国NACB准则，这里主要介绍神经元特异性烯醇化酶（NSE）、胃泌素释放肽前体 （ProGRP）、癌胚抗原（CEA）、细胞角蛋白19片段（CYFRA21-1）和鳞状细胞癌相关抗原（SCCA）等几种比较肯定的肿瘤标志物。 表1 国际上不同专家组对肺癌标志物应用的建议 1．神经元特异性烯醇化酶（neuron specific enol
15-3、CA19-9、PSA、SCC等），酶类（如神经元特异性烯醇化酶NSE、前列腺酸性磷酸酶PAP等），激素（hCG等），类固醇激素受体（如雌激素和孕酮受体等），单克隆免疫球蛋白和癌基因表达蛋白。
1.血清AFU升高主要见于原发性肝癌，与AFP联合检测，可提高肝癌的诊断阳性率。AFU活性高低与肝癌的大小和AFP浓度无明显相关，可作为原发性肝癌的早期诊断参考指标。肝癌术后观察血清AFU的水平，可用于监测疗效。 2.肺癌、乳腺癌、子宫癌以及肝硬化、糖尿病也可见升高。 3.妊娠期间，AFU升高，分娩后血清AFU迅速下降。 神经元特异性烯醇化酶(NSE) 正常情况：ELISA 0 ~ 12.5 μg/L，RIA 0.6 ~ 5.4 μg/L
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神经元特异性烯醇化酶(NSE)重组蛋白
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