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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 服务名称:
TRAP
- 提供商:
广州伯信生物科技有限公司
TRAP方法首先通过设计GST-MS2融合表达载体和ncRNA-MS2茎环结构串联重复载体,共转细胞获得GST-MS2融合蛋白和ncRNA-MS2融合RNA,在胞内形成GST-MS2~ncRNA-MS2与蛋白或RNA的复合体,最后裂解细胞,利用谷胱甘肽亲和琼脂糖珠子进行拉取,获得目的蛋白-RNA复合物,进一步分离纯化获得目的蛋白或目的RNA进行下一步检测分析。
- 研究RNA与蛋白、RNA或miRNA的互作;
- 使用伯信生物升级版环状RNA(circRNA)过表达载体,过表达效率高达1000倍以上!环化效率高达95%!
- 可以用于低丰度ncRNA分子功能研究,获得更多互作分子;
- 细胞内表达ncRNA分子结构及互作分子接近内源表达分子状态;
客户提供
① 新鲜组织:大于0.5g;
② 活细胞:大于107;
③ RNA信息、物种信息及ID;
伯信提供
① 实验报告(实验仪器、试剂、方法、结果、结论);
② MS搜库结果或western blot显影结果;
③ qPCR结果或测序结果。
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- 作者
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文献和实验The polymerase chain reaction (PCR) based telomeric repeat amplification protocol (TRAP) assay, to detect telomerase activity was originated by Kim et al. in late 1994 (1 ) and revolutionized the field of telomere/telomerase research
Gene-Trap Vectors and Mutagenesis
is performed with vectors that simultaneously inactivate and report the expression of the trapped gene and provide a molecular tag for its rapid identification. Gene-trap approaches have been used successfully in the past by both academic and commercial
Conditional Gene-Trap Mutagenesis in Zebrafish
the gene in all cells at all time, making it difficult to determine tissue-specific functions. We have adopted a FlEx Trap approach to generate conditional mutations in zebrafish by gene-trap mutagenesis. Combined with appropriate Cre or Flp lines
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