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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
TIMELESS Interacting Protein (TIPIN) (N-Term) 抗体
- 规格:
0.1ml/0.2ml
TIMELESS Interacting Protein (TIPIN) (N-Term) 抗体
anti-TIMELESS Interacting Protein (TIPIN) (N-Term) antibody
抗原
TIMELESS Interacting Protein (TIPIN)
别名 MGC82649, zgc:91928, 1110005A05Rik, 1110018P21Rik, RGD1564084, tipin
抗原表位
其他选择 N-Term
适用
其他选择 小鼠, 大鼠, 牛, 犬, 斑马鱼, 人, 小鸡
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 Western Blotting (WB)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 9至12个工作日
产品细节 TIMELESS Interacting Protein (TIPIN) (N-Term) 抗体
免疫原 Synthetic peptide directed towards the N terminal of human TIPIN
序列 MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA
预测反应 Bovine : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Dog : 90 %, Rat : 90 %, Chicken : 80 %, Zebrafish : 80 %
产品特性 This is a rabbit polyclonal antibody against TIPIN. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Protein A purified
目标详细情况 TIMELESS Interacting Protein (TIPIN) (N-Term) 抗体
别名 TIPIN
背景 TIPIN is required for normal progression of S-phase. It is important for cell survival after DNA damage or replication stress and may be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.
分子量 34 kDa
基因ID 54962
NCBI登录号 NM_017858, NP_060328
UniProt Q9BVW5
研究领域 Chromatin and Nuclear Signaling, DNA/RNA
使用细节 TIMELESS Interacting Protein (TIPIN) (N-Term) 抗体
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明
Antigen size: 301 AA
限制 仅限研究用
贮存及处理 TIMELESS Interacting Protein (TIPIN) (N-Term) 抗体
状态 Lyophilized
溶解方式 Add 100 µL of distilled water.
浓度 1 mg/mL
缓冲液 PBS buffer with 2 % sucrose
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For longer periods of storage, store at -20 °C
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文献和实验Identifying Interacting Proteins in an Escherichia coli-Based Two-Hybrid System
. In this system, a protein of interest (the bait) is fused to the full-length bacteriophage λ repressor protein (λcl), which consists of an amino-terminal DNA-binding domain and a carboxyl-terminal dimerization domain. Target proteins are fused to the N-terminal domain
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
pentagon, bait protein; open rectangle, noninteracting library protein; shaded box, activator protein (acid blob in Fig. ); open chevron, interacting library protein. View Image
Using the N Peptide to Tether Proteins to RNAs
bacteriophage antiterminator protein N (λN-(1–22) or λN peptide) is used to tag the protein of interest, and its specific 19 nt binding site (boxB) is inserted into the target RNA recruiting the properties of the fusion protein to the RNA
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