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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
P5CS protein (His-DHFR tag)
- 抗体英文名:
P5CS protein (His-DHFR tag)
- 克隆性:
多克隆
- 规格:
50µg
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文献和实验Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed inSaccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate
干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
CUT&Tag(Cleavage Under Targets and Tagmentation)是蛋白-DNA 互作的一大革新技术,它不需要使用甲醛交联以及免疫共沉淀,而是通过针对靶蛋白(如转录因子、染色质重塑蛋白)的抗体和 Protein A/G 的介导,使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头,经 PCR 扩增后即可形成用于高通量测序的文库(见图 1 )。CUT &Tag 技术具有细胞投入量低、信噪
Phos-tag Affinity Electrophoresis for Protein Kinase Profiling
Protein kinase profiling can provide a basis for understanding the molecular origins of diseases and, potentially, for developing tools for therapeutic intervention. It is therefore very important to develop advanced
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