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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) 抗体
- 规格:
0.1ml/0.2ml
SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) 抗体
anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody anti-SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) antibody (2)
抗原
SH3-Domain Binding Protein 1 (SH3BP1)
别名 SH3BP1, ARHGAP43, 3BP-1
抗原表位
其他选择 C-Term
适用
其他选择 人
宿主
其他选择 山羊
克隆类型
多克隆
标记
其他选择 非结合性
实验类型 Peptide ELISA
应用范围
其他选择 酶联免疫吸附测定(ELISA), Western Blotting (WB), 免疫组织化学(IHC)
Pubmed 2个引用可参考
规格 100 μg
发货至 中国 (更改)
发货时间 24至29个工作日
产品细节 SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) 抗体
免疫原 Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
纯化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
目标详细情况 SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) 抗体
别名 SH3BP1
背景 Synonyms: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
基因ID 23616
NCBI登录号 NP_061830
研究领域 Signaling
使用细节 SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) 抗体
应用备注 ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 µg/mL. Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).
限制 仅限研究用
贮存及处理 SH3-Domain Binding Protein 1 (SH3BP1) (C-Term) 抗体
状态 Liquid
缓冲液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Minimize freezing and thawing.
储存条件 -20 °C
储存方法 Aliquot and store at -20 °C.
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文献和实验独体—基于纤连蛋白类型Ⅲ结构域框架的抗体模拟 Akiko Koide and Shohei Koide
binding sites in proteins. Curr. Opin. Struct, Biol.7, 463-469. 3. Baron, M. , Norman, D. G. , and Campbell, I. D. ( 1 99 1 ) Protein modules. Trends Biochem. Sci. 16 , 13-17. 4. Bork, P. and Doolittle, R. F. ( 1992 )
of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248— 254. 40. Soldatov, A. V., Nabirochkina, E. N., Georgieva, S. G., and Eickhoff, H. (2001) Adjustment of transfer tools for the production of micro
dsDNA 微阵列又叫蛋白质结合微阵列(Protein binding microarray, PBM), 由Bulyk等[7]在1999 年首次提出。它的基本原理是在玻片上很小的面积内(约1 cm2)固定成千上万的dsDNA分子作为探针, 检测与大量DNA分子与DNA结合蛋白之间的相互作用。这种检测在原理上模拟的是DNA结合转录因子在体内与其DNA结合位点的相互作用。在基因组DNA中, 转录因子的DNA结合位点一般是一段很短(约5~20 bp)的序列, 如转录因子HIF-1的结合
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