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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPE189Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 25.4kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Gln454~Cys679 (Accession # Q8NBP7) with N-terminal His-Tag
前蛋白转化酶枯草溶菌素9(PCSK9)重组蛋白
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
QLFCRTV WSAHSGPTRM ATAVARCAPD EELLSCSSFS RSGKRRGERM EAQGGKLVCR AHNAFGGEGV YAIARCCLLP QANCSVHTAP PAEASMGTRV HCHQQGHVLT GCSSHWEVED LGTHKPPVLR PRGQPNQCVG HREASIHASC CHAPGLECKV KEHGIPAPQE QVTVACEEGW TLTGCSALPG TSHVLGAYAV DNTCVVRSRD VSTTGSTSEG AVTAVAICC
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
前蛋白转化酶枯草溶菌素9(PCSK9)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验动脉粥样硬化模型小鼠在是实验上存在一定的限制,如没有相关课题时需要一直保种饲养,随后,大量繁殖集齐数量足够年龄相近的小鼠也需要时间,近年来,科学家们建立了一种快速、简便、通用和经济有效的、利用病毒注射野生型小鼠的造模方式:AAV9-Pcsk9D377Y尾静脉注射。 Pcsk9D377Y过表达是近几年新出现的动脉粥样硬化急性模型之一。前蛋白转化酶枯草溶菌素转化酶9 (proprotein convertase subtilisin/kexin type 9, Pcsk9)主要由肝脏表达,介导
蛋白以及硫氧还蛋白(Trx)融合蛋白等。由于利用tag的特性通常可以对融合蛋白进行亲和层析等分离提纯,更多情况下选择融合表达是为了简化重组蛋白的纯化。因此出现两种融合表达类型:一是fusion tag位于目的蛋白的N端,这时tag可以提供良好表达所必需的信号,帮助提高目的蛋白的表达,缺点是纯化的表达产物中可能会有不完整的目的蛋白,原因是在翻译过程中意外中断的少量(C端)不完整的表达产物会一起被纯化。另一是fusion tag位于目的蛋白的C端,这可以保证只有完整的表达产物才会被纯化。当目的蛋白的功能
蛋白以及硫氧还蛋白(Trx)融合蛋白等。由于利用tag的特性通常可以对融合蛋白进行亲和层析等分离提纯,更多情况下选择融合表达是为了简化重组蛋白的纯化。因此出现两种融合表达类型:一是fusion tag位于目的蛋白的N端,这时tag可以提供良好表达所必需的信号,帮助提高目的蛋白的表达,缺点是纯化的表达产物中可能会有不完整的目的蛋白,原因是在翻译过程中意外中断的少量(C端)不完整的表达产物会一起被纯化。另一是fusion tag位于目的蛋白的C端,这可以保证只有完整的表达产物才会被纯化。当目的蛋白的功能
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