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- 技术资料
- 保存条件:
低温
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
100 Reactions
| M5132 | GoTaq® Hot Start Colorless Master Mix | 100 Reactions | 758 |
| M5133 | GoTaq® Hot Start Colorless Master Mix | 1,000 reactions | 5,779 |
| M5301 | M-MLV Reverse Transcriptase, RNase H Minus | 10000u | 818 |
| M5313 | M-MLV Reverse Transcriptase Buffer Pack | 2 × 1ml | 140 |
| M5761 | S1 Nuclease | 10,000u | 350 |
| M6101 | RQ1 RNase-Free DNase | 1,000u | 374 |
| M7122 | GoTaq® Green Master Mix | 100 reactions | 301 |
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文献和实验SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
an automatic “hot start” in PCR and increasing sensitivity, specificity, and yield. Pyrococcus species GB-D polymerase is a proofreading enzyme that possesses a 3’ to 5’ exonuclease activity. Mixture of this enzyme with Taq DNA polymerase results in a six-fold
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
,in which case, proceed to the next step. 5. Use 0.5 ml of product from the preceding step to template anested 50 ml PfuTurbo?hot start PCR in (see Note 13) 1×reaction buffer including 300 mM dNTPs, 3.75 U PfuTurbo® DNA Polymerase, and 250 nM GSP2 and GSP
Single tube confirmation PCR protocol
until the PCR is started. Alternatively, a "hot start" can be performed by adding the Taq polymerase to the individual tubes after the PCR mixtures have been heated to 94°C. Hot start improves the PCR results but this step is labor intensive
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