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- 2025年07月11日
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- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
CD247 Molecule (CD247) 抗体 (FITC)
- 规格：
0.1ml/0.2ml
CD247 Molecule (CD247)
别名
im:7156386, LOC100136514, CD3Z, LOC100136549, LOC100305023, CD247, 4930549J05Rik, A430104F18Rik, AW552088, Cd3, Cd3-eta, Cd3-zeta, Cd3h, Cd3z, Cd3zeta, T3z, Tcrk, Tcrz, CD3-ZETA, CD3H, CD3Q, T3Z, TCRZ ... show more
适用
其他选择 人, 小鼠
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (G3)
标记
其他选择 FITC
应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 找到1个引用
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb118360
产品细节 CD247 Molecule (CD247) 抗体 (FITC)
免疫原 113 Amino acid synthetic peptide corresponding to the cytoplasmatic domain of murine zeta chain. Spleen cells from immunised BALB/c mice were fused with cells of the Ag8.653 mouse myeloma cell line.
克隆位点 G3
亚型 IgG2a
特异性 A novel monoclonal antibody, named G3, has been developed that is specific for the TCR zeta chain subunit (CD247). The antibody is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain and may be used to detect TCR zeta expressing cells using flow cytometry on permeabilised cells.
纯化方法 Affinity chromatography on Protein A
目标详细情况 CD247 Molecule (CD247) 抗体 (FITC)
别名 CD247 / CD3Z
背景 The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.Synonyms: CD3 zeta chain, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3Z, TCRZ
基因ID 12503
NCBI登录号 NP_001106862
UniProt P24161
研究领域 CD Antigens, Surface Receptors of Immune Cells
使用细节 CD247 Molecule (CD247) 抗体 (FITC)
应用备注 Flow cytometry: Membrane permeabilisation is required for this application using a digitonin based method.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理 CD247 Molecule (CD247) 抗体 (FITC)
浓度 0.1 mg/mL
缓冲液 PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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文献和实验，用含有10%热灭活胎牛血清的RPMI1640培养基于5% CO2，37C条件下培养。抗CD20单克隆抗体HI47由我所免疫室提供。辣根过氧化酶标记羊抗人IgG，FITC标记的羊抗鼠IgG购于北京中山生物工程公司。2、抗CD20单克隆抗体轻、重链基因的克隆利用RT-PCR法，采用抗体通用的兼并性引物，从HI47杂交瘤细胞特异性地分别扩增抗体轻链、重链可变区基因（VL和VH）后，利用编码短肽（G4S）3的连接链DNA片段，经overlap PCR，将VH和VL基因连接构成ScFv基因（约750bp
the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labeled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other manufacturers may work equally well but have not been tested. ・ Screening cDNA Clones
5-hydroxytryptamine(serotonin) 5-羟色氨 HTLV human T-lymphocyte virus 人T-淋巴细胞病毒 LCAb islet cell antibody 胰岛细胞抗体 ICAM intercellular adhersion molecule 细胞间粘附因子 ICC immunocytochemistry 免疫细胞学 IDD insulin dependent diabetes 胰岛素依赖性糖尿病 IDRC (IRC
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