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- 2025年07月09日
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- 技术资料
- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
Lamin B1 (LMNB1) (C-Term) 抗体
- 规格：
0.1ml/0.2ml
Lamin B1 (LMNB1)
别名 MGC52550, Lamin-L(I), lamin-b, lmn, lmn2, lmnb, LMNB1, ADLD, LMN, LMN2, LMNB, fc06g01, wu:fc06g01
抗原表位
其他选择 C-Term
适用
其他选择 小鼠, 大鼠, 绵羊, 兔, 牛, 犬, 人
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (119D5-F1)
标记
其他选择 非结合性
应用范围
其他选择 Western Blotting (WB), Dot Blot (DB), 酶联免疫吸附测定（ELISA）, 流式细胞分析技术（FACS）, 免疫细胞化学（ICC）, 免疫萤光（IF）, 免疫组织化学（IHC）
Pubmed 2个引用可参考
规格 50 μL
发货至 中国 (更改)
发货时间 25至30个工作日
产品编号 yb108542
产品细节 Lamin B1 (LMNB1) (C-Term) 抗体
免疫原 Purified rat liver lamins
克隆位点 119D5-F1
亚型 IgG1 kappa
特异性 Reacts with an epitope located C-terminal of residue 231 in lamin B1. Lamins do not appear to be universally distributed among different cell and tissue types. ab8982 has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested
无交叉反应 斑马鱼
交叉反应 （详细） Does not react with Zebrafish
产品特性 Myeloma fusion partners:
P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c
Predicted molecular weight: 67 kDa.
纯化方法 Purified
目标详细情况 Lamin B1 (LMNB1) (C-Term) 抗体
别名 Lamin B1
背景 Synonyms:
Lamin B1 antibody,Lamin B2 antibody,LMN antibody,LMN B2 antibody,LMN2 antibody,LMNBantibody,LMNB1 antibody,LMNB2 antibody
UniProt P70615
研究领域 Signaling, Cytoskeleton, Apoptosis/Necrosis
使用细节 Lamin B1 (LMNB1) (C-Term) 抗体
应用备注 Optimal antibody dilution should be determined by titration, however as a guideline try, Flow Cyt: 1/100 - 1/200. ICC: 1/20 - 1/50. WB: 1/100 - 1/1000.
限制 仅限研究用
贮存及处理 Lamin B1 (LMNB1) (C-Term) 抗体
状态 Liquid
浓度 1 mg/mL
缓冲液 PBS containing 0.09 % sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件 4 °C
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文献和实验Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma
electrophoresis (2DE) and MALDI-TOF/TOF mass spectrometry, our groups have identified lamin B1 (LMNB1) and vimentin (VIM) as promising biomarkers for detection of early HCC. Protein levels of both biomarkers were significantly elevated in cancerous tissues
体系，显色体系等。beta Actin、beta Tubulin、GAPDH、Lamin B1、PCNA 等已作为成熟的内参运用到试验中，下面就大致介绍下如何选择内参：样本来源● 如果是哺乳动物的组织或细胞，主要以 beta Actin、beta Tubulin、GAPDH、Lamin B1、PCNA 为代表；● 如果是植物样本，主要以 actin、Rubisco 为代表。目的蛋白定位● 全细胞蛋白和细胞质：beta Actin、beta Tubulin、GAPDH 等；● 细胞核蛋白: PCNA
900200v Circulating Lamin B1 (LMNB1) Biomarker Detects Early Stages of Liver Cancer in Patients Stella Sun, Michelle Z. Xu, Ronnie T. Poon, Philip J. Day and John M. Luk DOI: 10.1021/pr9002118 First Insight into the Human Liver Proteome
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