- ￥739
- #R0560S
- 2026年01月04日
企业认证
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文献和实验Construction and Characterization of Adenovirus Vectors
using standard cloning procedures. Once obtained, the shuttle vector is linearized by digestion with PmeI and cotransformed into BJ5183 cells with the AdEasy backbone vector. After small-scale purification of DNA from BJ5183 cells
,7 PmeI GTTT/AAAC BbeI GGCGC/C PmlI CAC/GTG BbvCI CCTCAGC,-5,-2 PpiI GAA***NNNCTC,13,8 BclI T/GATCA Ppu10I A/TGCAT BfrBI ATG/CAT PpuMI RG/GWCCY BglI GC***NN/NGGC PshAI GA***/NNGTC Bpu10I CCTNAGC,-5,-2 PsiI TTA/TAA BsaAI YAC/GTR PspOMI G/GGCCC BsaHI
Linearization and Purification of the Shuttle Plasmid
water 3.0 Procedure 3.1 Linearization 3.1.1 Linearize shuttle plasmid with either PmeI, NheI, SwaI, or SfiI. Make sure the enzyme you choose does not cut in your insert. 3.1.2 Run sample on gel to confirm complete
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