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- 2026年01月27日
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文献和实验Ahr knockout genotyping by PCR
the fragment with Eco47III, HaeII, or HhaI. (FYI-HaeII and HhaI are much less expensive than Eco47III.) The “d” allele does not cut with the enzyme due to a polymorphism at that site, but the b1 allele cuts once to yield 142bp and 76bp fragments. The Ahr
Clonality - X Chromosome Inactivation Assay
and can assist in interpretation of allele patterns. 4. References Allen RC, Zoghbi HY, Moseley AB, Rosenblatt HM, Belmont JW. Methylation of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome
Denaturing Gradient Gel Electrophoresis (DGGE)
(which may occur as frequently as every 400 bp). Genomic DNA is digested with an enzyme combination that cuts the DNA into fragments of average size 200-700 bp. Enzymes with 4-base recognition sites, such as AluI, HaeIII, HhaI, MspI, and RsaI are the most useful
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