D-葡萄糖胺检测试剂盒

UV-method for the determination of D-Glucosamine,
D-Glucosamine sulphate and N-Acetyl Glucosamine in food
supplements, foodstuffs, beverages and other materials

Principle:
(desulphation)
(1) Glucosamine sulphate → D-glucosamine + sulphate

(deacetylation)
(2) N-Acetyl Glucosamine → D-glucosamine + acetate

(hexokinase)
(3) D-Glucosamine + ATP → D-glucosamine-6-P + ATP

(glucosamine 6-phosphate deaminase)
(4) D-Glucosamine-6-P + H₂O → D-fructose-6-P + NH₄⁺

(phosphoglucose isomerase)
(5) F-6-P → G-6-P

(glucose-6-phosphate dehydrogenase)
(6) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

Kit size: 50 assays (manual) / 500 (microplate)
/ 500 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 8 min
Detection limit: 1.33 mg/L
Application examples:
Food supplements, food products and beverages
Method recognition: Novel method