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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Myeloid Differentiation Factor 88 (MyD88)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. RPB707Mu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 28.4kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Arg32~Pro265 (Accession # P22366) with N-terminal His-Tag
髓分化因子88(MyD88)重组蛋白
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- RLSLFLNPR TPVAADWTLL AEEMGFEYLE IRELETRPDP TRSLLDAWQG RSGASVGRLL ELLALLDRED ILKELKSRIE EDCQKYLGKQ QNQESEKPLQ VARVESSVPQ TKELGGITTL DDPLGQTPEL FDAFICYCPN DIEFVQEMIR QLEQTDYRLK LCVSDRDVLP GTCVWSIASE LIEKRCRRMV VVVSDDYLQS KECDFQTKFA LSLSPGVQQK RLIPIKYKAM KKDFP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
髓分化因子88(MyD88)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验小鼠髓样分化因子88(MyD88)酶联免疫分析(ELISA)
小鼠髓样分化因子88(MyD88) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及相关液体样本中 髓样分化因子88(MyD88) 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 小鼠髓样分化因子88(MyD88) 水平。用纯化的 小鼠髓样分化因子88(MyD88) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 髓样分化因子
zhang7513 请问各位大侠! Myd88代表什么意思?请指教!谢谢! 77472087 髓样分化蛋白-88 myeloid differentiation factor 88, MyD88 happylife004007 髓样分化蛋白88, 它是一种接头蛋白分子,含有TIR结构域,在天然免疫中介导信号传导。 zhouasd 细胞分化因子88
。 固有免疫中针对TLR信号转导的反馈调节 ASK1:凋亡信号调节激酶1; IRAK:IL-1受体相关激酶; MAPK:丝裂原激活蛋白激酶; MyD88:髓样分化因子88; NF-κD:核因子;Rac: 小G蛋白; SIGIRR:单一IgIL-1R相关分子; TIRAP:TIR(To11/IL-1受体)相关蛋白; TRAF6:TNF受体相关因子6。 在这些负向调节因子的作用下,可构成针对PAMP特别是其中I。PS应答的无反应期,防止因细胞因子过度产生而引起
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