- ¥5841
- promega
- 进口
- E2820
- 2025年12月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
1,000 assays
| E2820 | Renilla Luciferase Assay System | 1,000 assays | 5,841 |
| E2920 | Dual-Glo® Luciferase Assay System | 10ml | 2,179 |
| E2940 | Dual-Glo® Luciferase Assay System | 100ml | 12,887 |
| E2980 | Dual-Glo® Luciferase Assay System | 10 × 100ml | 114,175 |
| E3030 | Primer Extension System-AMV Reverse Transcriptase | 40 reactions | 1,617 |
| E3050 | Gel Shift Assay Core System | 100 reactions | 2,830 |
| E3091 | HeLaScribe® Nuclear Extract in vitro Transcription Grade | 40 reactions | 3,456 |
| E3092 | HeLaScribe® Nuclear Extract in vitro Transcription Grade | 160 reactions | 9,302 |
| E3110 | HeLaScribe® Nuclear Extract in vitro Transcription System | 40 reactions | 5,004 |
| E3201 | AP1 Consensus Oligonucleotide | 175pmol | 563 |
| E3202 | AP1 Consensus Oligonucleotide | 35pmol | 303 |
| E3211 | AP2 Consensus Oligonucleotide | 175pmol | 563 |
| E3212 | AP2 Consensus Oligonucleotide | 35pmol | 303 |
| E3221 | TFIID Consensus Oligonucleotide | 175pmol | 563 |
| E3222 | TFIID Consensus Oligonucleotide | 35pmol | 303 |
| E3231 | SP1 Consensus Oligonucleotide | 175pmol | 563 |
| E3232 | SP1 Consensus Oligonucleotide | 35pmol | 303 |
| E3241 | OCT1 Consensus Oligonucleotide | 175pmol | 563 |
| E3242 | OCT1 Consensus Oligonucleotide | 35pmol | 303 |
| E3281 | CREB Consensus Oligonucleotide | 175pmol | 563 |
| E3282 | CREB Consensus Oligonucleotide | 35pmol | 303 |
| E3291 | NF-κB Consensus Oligonucleotide | 175pmol | 563 |
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文献和实验The Split Luciferase Complementation Assay
A split luciferase complementation assay to study protein–protein interactions within Arabidopsis protoplasts in 96-well plates is described in this protocol. Two proteins of interest, a bait and prey, which are genetically fused to amino
superskyfly 我自己都觉得我自己很牛，千百人都做出来的试验，我就是做不出来。质粒用的是Promega p4.32-NF-kB-Luc, 转染用的是Invitrogen的Lipofactamin, 激活用的是Sigma生产的PMA和PHA-P,Assay用的是Promega dual,结果加了PMA和PHA的和不加的读数几乎没有区别。加了compound的和不加的也没有区别。 用过PC3, Jurkat, A549，HCT116都是一样。
Luciferase Assay to Study the Activity of a Cloned Promoter DNA Fragment
cellular contexts, and for dissecting binding elements in the promoter. Here, we describe the use of the Dual-Luciferase� Reporter Assay System created by Promega (Promega Corporation, Wisconsin, USA) to study the cloned 6.7 kilobases (kb) mouse (m) Tcf
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