Heat Shock Protein 70 (HSP70)

Heat Shock Protein 70 (HSP70) 抗体 (FITC)抗原
Heat Shock Protein 70 (HSP70)
别名
HSP70, HEAT SHOCK PROTEIN 70, HEAT SHOCK PROTEIN 70-7, HSC70-7, K9P8.5, K9P8_5, chloroplast heat shock protein 70-2, cpHsc70-2, F19K16.12, F19K16_12, LOC100305036, hsc70, CG31354, Hsp70Bb, hsp70B, hsp ... show more
适用
其他选择 人, 大鼠, 牛, 犬, 小鸡, Fruit Fly (Drosophila melanogaster), Crab, 豚鼠, Hamster, 猴, 猪, 兔, 绵羊
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (C92F3A-5)
标记
其他选择 FITC
应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 14个引用可参考
规格 50 μg
发货至 中国 (更改)
发货时间 24至29个工作日
产品编号 yb361671
产品细节 Heat Shock Protein 70 (HSP70) 抗体 (FITC)
免疫原 Human Hsp70
克隆位点 C92F3A-5
亚型 IgG1
特异性 Detects a approx. 70 kDa protein corresponding to the molecular mass of inducible Hsp70 on SDS PAGE immunoblots. The mapped epitope is in the region of AA 436-503.
交叉反应 （详细） Does not cross-react with Hsc70 (Hsp73).
灵敏度 100 µg/mL of SMC-103 was sufficient for detection of Hsp70 in human Jurkat cells by FACS analysis.
纯化方法 Protein G Purified
目标详细情况Heat Shock Protein 70 (HSP70) 抗体 (FITC)
别名 Hsp70
背景 Synonyms:
Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, Hsp73, HSPA1, HSPA1A, HSPA1B
Hsp70 genes encode abundant heat-inducible 70 kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O % identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteinsand synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins.All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
基因ID 3303
NCBI登录号 NP_005336
UniProt P08107
研究领域 Cancer, Heat Shock Proteins, Signaling
使用细节 Heat Shock Protein 70 (HSP70) 抗体 (FITC)
应用备注 Recommended Dilution: 1:1000
限制 仅限研究用
贮存及处理 Heat Shock Protein 70 (HSP70) 抗体 (FITC)
浓度 0.33 mg/mL
缓冲液 PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
储存条件 4 °C