- 询价
- 沪震生物
- 进口/国产
- hz21Hu01
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant C-Type Natriuretic Peptide (CNP)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPA721Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 11.3kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
C-型钠尿肽(CNP)重组蛋白
Subcellular Location n/a
Residues Arg33~Gly125 (Accession # P23582) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- RTPPAEEL AEPQAAGGGQ KKGDKAPGGG GANLKGDRSR LLRDLRVDTK SRAAWARLLQ EHPNARKYKG ANKKGLSKGC FGLKLDRIGS MSGLG
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
C-型钠尿肽(CNP)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验小鼠C型钠尿肽(CNP) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本 中 C型钠尿肽(CNP) 的 含量。 实验原理 ： 本试剂盒应用 双抗体 夹心法测定 标本 中 小鼠C 型钠尿肽 (CNP) 水平。用纯化的 小鼠C 型钠尿肽 (CNP) 抗体 包被微孔板，制成固相 抗 体，往包被 单抗 的微孔中依次加入 C型钠尿肽 (CNP
the reconstituted vial to sit at 4oC for at > 2 hours before use. (将重悬液在4oC静置2小时以上) 3. This solution can be stored at 2-8oC for up to 1 week. 第 3 步：该重悬液在 2-8oC 最长可保存 1 周。 用说明书上推荐的溶液将细胞因子或重组蛋白重悬到推荐的浓度后，细胞因子或重组蛋白可放置在2-8o C，即冰箱的冷藏室。在这种条件下，细胞因子或重组蛋白的活性
can be stored at 2-8oC for up to 1 week. 第 3 步：该重悬液在 2-8oC 最长可保存 1 周。 用说明书上推荐的溶液将细胞因子或重组蛋白重悬到推荐的浓度后，细胞因子或重组蛋白可放置在2-8o C，即冰箱的冷藏室。在这种条件下，细胞因子或重组蛋白的活性最长可保持1周。这对一个周期为5-7天的实验，如DC(树突状细胞)的诱导成熟是足够的。在此期间，只要每次从冰箱里吸取一定量的细胞因子或重组蛋白溶液加入到培养体系内即可。 其实，说明书上推荐的浓度
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