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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Homing Associated Cell Adhesion Molecule (HCAM)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. RPA670Mu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass n/a
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
归巢关联细胞黏附分子(HCAM)重组蛋白 Subcellular Location n/a
Residues n/a
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
归巢关联细胞黏附分子(HCAM)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验[188];(6)与分子伴侣共表达[189];(7)与T4 pin基因共表达[190];(8)替换特定的氨基酸残基以消除蛋白酶裂解位点[191];(9)改善蛋白质的亲水性[192];(10)优化培养条件[193]融合蛋白表达 在E.coli中表达外源蛋白,尤其是真核蛋白时,蛋白质的稳定性是经常遇到的问题。最近几年,众多巧妙的蛋白质——融合系统的发展,为E.coli中高效表达和纯化重组蛋白提供了极大方便。融合表达具有多方面的优点:如防止包涵体的形成,促进蛋白质的正确折叠,限制蛋白酶解和利于纯化
程度的重叠与区别。迄今为止,外泌体的分离方法尚无公认的“金标准”。由于技术的限制,无论采用何种方法,都无法将外泌体与其他EV 亚群彻底分离,因此,目前在递送载体方面的研究和应用中,外泌体常与其他 EV 亚群混合使用。哺乳动物和植物细胞均能产生外泌体。外泌体是一个具有高度异质性的群体,其异质性表现在多个方面,包括大小异质性、包含物异质性、功能异质性和细胞来源异质性。不同细胞来源的外泌体其大小、表面受体和内容物往往具有不同特征。细胞靶向分子、细胞黏附分子、代谢物和核酸等在外泌体的形成过程中被包裹在囊泡腔内
院上海逆境生物学研究中心徐通达教授课题组发表了题为“TMK1-mediated auxin signalling regulates differential growth of the apical hook”的文章,报道了植物类受体蛋白激酶Transmembrane Kinase 1(TMK1)介导的生长素信号途径调控植物差异性生长的分子机制。②基因编辑技术的潜在危害——可产生抗性病毒根据阿尔伯塔大学(University of Alberta)、比利时列日大学(University
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