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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
Exosome Component 6 (EXOSC6) (N-Term) 抗体
- 规格:
0.1ml/0.2ml
Exosome Component 6 (EXOSC6)
别名 exosc6, RAR-gamma, id:ibd1130, wu:fe17d05, zgc:110071, EAP4, MTR3, Mtr3p, hMtr3p, p11, 2610510N21Rik, C76919, Mtr3
抗原表位
其他选择 N-Term
适用
其他选择 犬, 大鼠, 小鼠, 牛, 人
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 免疫组织化学(IHC), Western Blotting (WB)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 9至12个工作日
产品编号 yb184073
产品细节 Exosome Component 6 (EXOSC6) (N-Term) 抗体
免疫原 Synthetic peptide directed towards the N terminal of human EXOSC6
序列 MPGDHRRIRG PEESQPPQLY AADEEEAPGT RDPTRLRPVY ARAGLLSQAK
预测反应 Bovine : 92 %, Rat : 92 %
产品特性 This is a rabbit polyclonal antibody against EXOSC6. It was validated on Western Blot and immunohistochemistry.
纯化方法 Protein A purified
目标详细情况 Exosome Component 6 (EXOSC6) (N-Term) 抗体
别名 EXOSC6
背景 EXOSC6 constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.This gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.
分子量 30 kDa
基因ID 118460
NCBI登录号 NM_058219, NP_478126
UniProt Q5RKV6
研究领域 Chromatin and Nuclear Signaling, DNA/RNA
使用细节 Exosome Component 6 (EXOSC6) (N-Term) 抗体
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明
Antigen size: 272 AA
限制 仅限研究用
贮存及处理 Exosome Component 6 (EXOSC6) (N-Term) 抗体
状态 Lyophilized
溶解方式 Add 100 µL of distilled water.
浓度 1 mg/mL
缓冲液 PBS buffer with 2 % sucrose
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For longer periods of storage, store at -20 °C
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文献和实验(electrophoretic mobility shift assay),DNase I 足印法(DNase I footprinting),酵母单杂交系统等。但这些方法都有一定的局限性,不能充分反映生理情况下DNA与蛋白相互作用的真实情况,而且很难捕捉到在染色质水平上基因表达调控的动态瞬时事件[2]。 染色质免疫沉淀技术(Chromatin Immunoprecipitation,简称ChIP)是研究体内蛋白质与DNA相互作用的一种技术。它利用抗原抗体反应的特异性,可以真实地反映体内蛋白因子与基因组DNA结合
网络 免疫球蛋白分子的结构与功能 一、免疫球蛋白分子的基本结构 Porter等对血清IgG抗体的研究证明,Ig分子的基本结构是由四肽链组成的。即由二条相同的分子量较小的肽链称为轻链和二条相同的分子量较大的肽链称为重链组成的。轻链与重链是由二硫键连接形成一个四肽链分子称为Ig分子的单体,是构成免疫球蛋白分子的基本结构。Ig单体中四条肽链两端游离的氨基或羧基的方向是一致的,分别命名
佚名 Porter等对血清IgG抗体的研究证明,Ig分子的基本结构是由四肽链组成的。即由二条相同的分子量较小的肽链称为轻链和二条相同的分子量较大的肽链称为重链组成的。轻链与重链是由二硫键连接形成一个四肽链分子称为Ig分子的单体,是构成免疫球蛋白分子的基本结构。Ig单体中四条肽链两端游离的氨基或羧基的方向是一致的,分别命名为氨基端(N端)和羧基端(C端
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