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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Bcl2 Like Protein 11 (BCL2L11)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPQ166Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 17.3kDa
Predicted isoelectric point 6.8
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location Mitochondrion.
Residues Ala2~Arg120 (Accession # O43521) with two N-terminal Tags, His-tag and T7-tag
Bcl2样蛋白11(BCL2L11)重组蛋白Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AKQPSDVSS ECDREGRQLQ PAERPPQLRP GAPTSLQTEP QDRSPAPMSC DKSTQTPSPP CQAFNHYLSA MASMRQAEPA DMRPEIWIAQ ELRRIGDEFN AYYARRVFLN NYQAAEDHPR
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Bcl2样蛋白11(BCL2L11)重组蛋白Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
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文献和实验(HIC)反相(RPC) 特异性结合 亲和(AC) 每一种方法都有分辨率、处理量、速度和回收率之间的平衡。 分辨率:由选择的方法和层析介质生成窄峰的能力来实现。总的来说,当杂质和目标蛋白性质相似时,在纯化的最后阶段分辨率是重要因素。 处理量:一般指在纯化过程中目标蛋白的上样量。如上样体积、浓度等。 速度:在初纯化中是重要
编号:200-13)的说明书中您会看到:Slow to dissolve (缓慢溶解),在重组人IL-11(产品编号:200-11)的说明中会看到: This solution is slow to dissolve.(该溶液溶解缓慢)。这种情况下,您最好能将要溶解的细胞因子或重组蛋白放在水平摇床(shaker)上低速摇一段时间,促使细胞因子或重组蛋白完全溶解。 有些蛋白,如重组人IL-13 Variant (产品编号:200-13A)虽然难溶,但却不需用摇床来加速溶解。其说明书上的备注
您会看到:Slow to dissolve (缓慢溶解),在重组人IL-11(产品编号:200-11)的说明中会看到: This solution is slow to dissolve.(该溶液溶解缓慢)。这种情况下,您最好能将要溶解的细胞因子或重组蛋白放在水平摇床(shaker)上低速摇一段时间,促使细胞因子或重组蛋白完全溶解。 有些蛋白,如重组人IL-13 Variant (产品编号:200-13A)虽然难溶,但却不需用摇床来加速溶解。其说明书上的备注为:Allow
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