2-Nitrophenyl β-D-galactopyran

2-Nitrophenyl β- D -galactopyranoside

≥98% (enzymatic)

产品名称
General description
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

包装
1 g in poly tube

250 mg in poly bottle

5, 25 g in poly bottle

500 mg in poly tube

Principle
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Reconstitution
A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.
性质
Related Categories Chromogenic, Galactoside, Gene Reporter Substrates, Inhibitors and Substrates, Media Components,
Substrates, Transformation of Yeast, 克隆和表达, 分子生物学, 分子生物学和蛋白质表达用微生物培养基, 糖生物学, 翻译后修饰, 蛋白质组学
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grade for molecular biology
assay ≥98% (enzymatic)
form powder
storage temp. −20°C