凝血酶 来源于人类血浆

凝血酶 来源于人类血浆

Thrombin from human plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E 1%/280 , 18.3)

产品名称
Frequently Asked QuestionsLive Chat andFrequently Asked Questions are available for this Product.Analysis NoteNIH 试验步骤基于 Biggs 方法的改进，使用 0.2mL 稀释血浆（与生理盐水 1:1 混合，作为底物）和 0.1mL Biggs 凝血酶样品（稳定于 1% 缓冲白蛋白溶液中）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。Biochem/physiol Actions丝氨酸蛋白酶，可选择性裂解血纤维蛋白原的 Arg-Gly 键，形成血纤维蛋白和血纤维蛋白肽 A 和 B。General descriptionThrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.Other Notes查看更多关于凝血酶的信息，请访问www.sigma-aldrich.com/enz​ymeexplorer。Unit DefinitionActivity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference StandardPhysical formLyophilized from saline sodium citrate buffer, pH 6.5ApplicationThrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to investigate the protein C pathway in intestinal barrier function.1
性质
Related Categories3.4.x.x Peptidases,3.x.x.x Hydrolases,Application Index,Coagulation Proteins and Reagents,Core Bioreagents,
Enzyme Class Index,Enzymes,Plasma & Blood Proteins,Plasma, Blood, and Related Proteins and Reagents,Proteases,Proteases & Protein Sequencing,Proteases for Recombinant Protein Fusion Tag Cleavage,Proteolytic Enzymes,Proteolytic Enzymes and Substrates,Research Essentials,Selective Proteolytic Enzymes,Serum Proteins and Related Enzymes,Serum Related Enzymes,Thrombin,生化试剂,蛋白质及衍生物,酶、抑制剂和底物更多...form lyophilized powderimpurities HIV and HBsAg, source material tested negativestorage temp. −20°CGene Information human ...F2(2147)